Objective To realize hand hygiene status and effect of hand-drying measures on hand-washing of health care workers(HCWs)in an intensive care unit(ICU).Methods From February to April 2013,210 HCWs in an ICU were selected and randomly divided into three groups,group A dried hands with paper towel,group B with hand drier,and group C with personal towel,specimens from hands before hand-washing,after hand-washing,and after hand-drying were taken and performed detection.Results Hand microorganism count in group A,B and C before washing hands after contacting patients was (29.10±15.08)CFU/cm2 ,(31.42±14.76)CFU/cm2 and (30.36±15.52) CFU/cm2 respectively,the difference was not statistically different(F=0.048,P >0.05);After six-step hand-washing, hand microorganism count before hand drying in group A,B and C was (3.26 ±0.61 )CFU/cm2 ,(2.98 ±0.59) CFU/cm2 and (3.87±0.67)CFU/cm2 respectively,compared with hand microorganism count before hand-washing, the difference was statistically different(all P <0.01 ).After adopting different hand-drying methods,microorgan-ism count in three groups was statistically different(F =1 .892,P <0.05),group A ([1 .29±0.58]CFU/cm2 )was significantly lower than group B and C,group B ([9.51 ±0.73 ]CFU/cm2 )was significantly lower than group C ([22.76±4.11]CFU/cm2 );the qualified rate in group A (90.00%)was significantly higher than group B and C, group B (68.57%)was significantly higher than group C (47.14%).The top 5 pathogens isolated from HCWs’ hands were Pseudomonas aeruginosa ,Acinetobacter baumannii ,Klebsiella pneumoniae ,Escherichia coli ,and Coag-ulase negative Staphylococcus ,these strains were highly consistent with the top 5 multidrug-resistant organisms (MDROs)isolated from ICU patients in this hospital in 2013.Conclusion HCWs’hands are seriously contamina-ted after all kinds of medical performance;if hands are improperly dried,secondary contamination may occur;patho-gens isolated from HCWs’hands are highly consistent with MOROs from patients,timely and correct hand-washing and hand-drying is the key link to ensure the quality of hand hygiene,and is of great significance to reduce the occur-rence of MDROs infection in ICU patients.%目的：了解重症监护室（ICU）医务人员手卫生状况及干手措施对洗手效果的影响。方法选择2013年24月某院 ICU 医务人员210人次，将其随机分为 ABC 三组，A 组采用干手纸巾法、B 组采用干手器法、C 组采用个人小毛巾法干手，分别对其洗手前、洗手后和干手后进行手卫生现场采样检测。结果A、B、C 3组医务人员接触患者后洗手前手部菌落数分别为（29．10±15．08）CFU/cm2、（31．42±14．76）CFU/cm2和（30．36±15．52） CFU/cm2，3组比较，差异无统计学意义（F ＝0．048，P ＞0．05）；按六步洗手法洗手后湿手采样，医务人员手部菌落数分别为（3．26±0．61）CFU/cm2、（2．98±0．59）CFU/cm2和（3．87±0．67）CFU/cm2，与洗手前比较，差异均有统计学意义（均 P ＜0．01）。采用不同的干手方式干手后，3组医务人员手部菌落数比较，差异有统计学意义（F ＝1．892，P ＜0．05）；A 组菌落数[（1．29±0．58）CFU/cm2]低于 B、C 组，B 组[（9．51±0．73）CFU/cm2]低于 C 组[（22．76±4．11）CFU/cm2]；A 组合格率（90．00％）高于 B、C 组，B 组合格率（68．57％）高于 C 组（47．14％）。医务人员手分离病原菌居前5位的是铜绿假单胞菌、鲍曼不动杆菌、肺炎克雷伯菌、大肠埃希菌和凝固酶阴性葡萄球菌，与2013年度该院 ICU 分离居前5位的多重耐药菌高度一致。结论ICU 医务人员进行各种诊疗护理操作后，手部污染严重；若干手方法不当，会导致手二次污染；其手分离的病原菌与患者分离的多重耐药菌高度一致。及时、正确地洗手和干手是保证手卫生质量的关键环节，对降低 ICU 患者多重耐药菌感染具有重要意义。
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