HMGB1抗体对刀豆蛋白A引起小鼠肝损伤的保护作用

摘要

Objective To detect the protective role of high mobility group box-1 protein (HMGB1 )antibody in concanavalin A(ConA)-induced liver injury in mice.Methods The healthy male Balb/c mice were grouped into con-trol group (saline injection),model group(ConA injection)and experimental group(ConA+HMGB1 antibody injec-tion).After 6 hours of injection,mice blood was collected for detecting alanine transaminase (ALT)and HMGB1 , liver tissue was used to do HE stain,Tunel,and immunofluorescence detection.Results Pathological inflammation in experimental group was slighter than model group.The levels of ALT and HMGB1 in mice serum were (52.00± 8.34)U/L and (7.54 ±0.53)ng/mL in control group,(5 551 .50 ±1 445.74)U/L and (18.06 ±1 .65 )ng/mL in model group,(1 977.40±654.89)U/L and (10.77±0.71)ng/mL in experimental group,respectively;the expres-sion levels of HMGB1 mRNA and HMGB1 (relative value)in liver tissue were 1 .886±0.253 and 0.086±0.028 in control group,4.718±0.341 and 0.268±0.043 in model group,3.005 ±0.331 and 0.116±0.008 in experimental group,respectively;the expression levels of ALT and HMGB1 in serum,as well as HMGB1 mRNA and HMGB1 in liver tissue of experimental group were all lower than model group(all P <0.001).Apoptosis and HMGB1 migra-tion in the liver cell (normalized)were 1 ±0 and 1 ±0 in control group,4.67 ±0.33 and 4.50 ±0.22 in model group,2.67±0.21 and 2.33 ±0.21 in experimental group,respectively;apoptosis and HMGB1 migration in liver tissue of experimental group were both lower than model group(both P <0.001).Conclusion HMGB1 antibody can improve the pathological injury of liver tissue,and protect mice liver against the injury induced by ConA.%目的：研究高迁移率族蛋白1（HMGB1）抗体对刀豆蛋白 A（ConA）引起小鼠肝损伤的保护作用。方法将健康无污染雄性 Balb／c 小鼠分成空白对照组（注射生理盐水）、模型组（注射 ConA）和实验组（注射 ConA＋HMGB1抗体），3组小鼠注射药物6 h 后摘眼球取血，所获血液离心后取血清检测谷丙转氨酶（ALT）和HMGB1，留取肝脏组织进行 HE 染色、Tunel、免疫荧光等检测。结果实验组小鼠肝组织病理炎症反应轻于模型组。小鼠血清中 ALT、HMGB1：空白对照组分别为（52．00±8．34）U／L、（7．54±0．53）ng／mL，模型组分别为（5551．50±1445．74）U／L、（18．06±1．65）ng／mL，实验组分别为（1977．40±654．89）U／L、（10．77±0．71）ng／mL；肝组织中 HMGB1 mRNA、HMGB1表达量（相对值）：空白对照组分别为1．886±0．253、0．086±0．028，模型组分别为4．718±0．341、0．268±0．043，实验组分别为3．005±0．331、0．116±0．008；实验组小鼠血清中 ALT、HMGB1，以及肝组织中 HMGB1 mRNA、HMGB1表达量均低于模型组（均 P ＜0．001）。肝组织细胞凋亡、肝细胞内 HMGB1迁移（标准化）：空白对照组均为1±0，模型组分别为4．67±0．33、4．50±0．22，实验组分别为2．67±0．21、2．33±0．21；实验组小鼠肝组织细胞凋亡、肝细胞内 HMGB1迁移均低于模型组（均 P ＜0．001）。结论HMGB1抗体能改善肝组织的病理损伤，可以保护 ConA 引起的小鼠肝损伤。