2008-2011年北京协和医院流感嗜血杆菌耐药性监测

摘要

Objective To investigate the antimicrobial resistance of Haemophilus influenzae in Peking Union Medical College Hospital for rational clinical treatment.Methods A total of 223 strains of H .influenzae were collected from patients from Jan-uary 2008 to December 2011.The antimicrobial susceptibility was tested by Kirby-Bauer method.Production of beta-lactamase was detected using nitrocefin disks.WHONET 5.6 software was used to analyze the data of susceptibility testing.Results Tri-methoprim-sulfamethoxazole and tetracycline were the two antimicrobial agents to which the H .influenzae strains were most resistant.Theβ-lactamase positive ampicillin resistant strains accounted for 15.9 %,andβ-lactamase negative ampicillin resist-ant strains accounted for 8.9%.Conclusions H .influenzae is mainly isolated from respiratory specimens.Majority of the anti-microbial agents still show good antibacterial activity against H .influenzae strains.However,H .influenzae isolates are highly resistant to trimethoprim-sulfamethoxazole and tetracycline.%目的：了解北京协和医院临床分离的流感嗜血杆菌的耐药情况，为临床治疗该菌感染提供参考依据。方法收集2008年1月至2011年12月223株临床分离的流感嗜血杆菌，用纸片扩散法进行抗菌药物敏感试验，并用 Nitrocefin 纸片检测β内酰胺酶，用 WHONET 5．6软件对流感嗜血杆菌耐药率进行统计分析。结果流感嗜血杆菌对抗菌药物耐药率居前2位的是四环素和甲氧苄啶-磺胺甲口恶唑。β内酰胺酶阳性氨苄西林耐药的菌株35株（15．7％），β内酰胺酶阴性氨苄西林耐药的菌株20株（9．0％）。结论流感嗜血杆菌主要分离自呼吸道标本，大多数抗菌药物对流感嗜血杆菌仍然保持较好的抗菌活性，但该菌对甲氧苄啶-磺胺甲口恶唑和四环素的耐药率最高。