长波紫外线对大鼠皮肤角朊细胞的损伤及茶多酚的保护作用

摘要

Objective To study the effects of UVA and tea polyphenols(TPP,a kind of polyphenol substances from a special plant) on lipid peroxidation and cell kinetics in primarily cultured rat skin keratinocytes.Methods The release of cytoplasm enzyme, lactate dehydrogenase( LDH), lipid peroxidation product, malondialdehyde (MDA) ,and anti-peroxidant, glutathione peroxidase(GSH-Px) ,were assayed in primary cultured keratinocytes treated by UVA and TPP. Cytometry and Flow Cytometer were used to count the viable cells and the cell kinetics,too. Results UVA could obviously improve the release of LDH from 827.55 U/L to 1 312.47 U/L,increase the formation of peroxidation product MDA,decrease the level of GSH-Px and reduce the survival rate of cells and the cell proliferative index( PI) from 34.24% to 17.98 %. TPP could significantly protect and inhibit the damage induced by UVA. Conclusion UVA could damage skin keratinocytes, and TPP could protect keratinocytes from damage induced by UVA. The result could provide useful information in developing natural anti-violet product.%目的探讨长波紫外线(uVA)对原代培养的大鼠皮肤角朊细胞脂质过氧化和生长状况等影响，同时探讨一种植物多酚—茶多酚(TPP)在此过程中所起的作用。方法在原代培养大鼠皮肤角朊细胞基础上，经UVA照射后，测定角朊细胞胞浆酶—乳酸脱氢酶(LDH)释放情况，脂质过氧化产物丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)水平，并测定培养细胞的存活率和细胞周期动力学。结果UVA可以引起体外培养的角朊细胞膜通透性增强，胞浆酶LDH释放增加(从827．55 U／L增至1 312．47 U／L)；脂质过氧化产物MDA水平升高，抗氧化酶GSH-Px水平降低；细胞存活率下降，细胞周期动力学表现为细胞增殖抑制：增殖指数(PI)从34．24％降至17．98％。天然提取物TPP(质量浓度为0．1％)可以比较明显地抑制UVA引起的上述损害。结论UVA可损伤原代培养的大鼠皮肤角朊细胞，TPP为天然防晒产品的开发提供理论依据。