肝脏中乙醇脱氢酶活力在大蒜油阻止大鼠正己烷神经损伤中的变化

摘要

Objective To study the protective effects of garlic oil (GO) on the peripheral nerve injuries induced by n-hexane.Methods Male Wistar rats were randomly divided into four groups ( 10 rats in each group):the control, the n-hexane treatment ( 2000 mg/kg), the low dose GO, and the high dose GO groups.The rats in the low and high doses of GO groups were pretreated with GO (40 and 80 mg/kg) before exposure to n-hexane (2000 mg/kg ), while the animals of the n-hexane treatment group were given normal saline and then 2000 mg/kg n-hexane.The rats were exposed to GO and n-hexane 6 times a week for 10 weeks.The gait scores and staying time on the rotating rod for all rats were detected every two weeks.The rats were sacrificed at the end of ten weeks,then the levels of alcohol dehydrogenase (ADH), maleic dialdehyde (MDA), reduced glutathione OH in livers were examined.Results The gait scores increased significantly and the time staying on the rotating rod obviously decreased in rats of n-hexane treatment group, as compared with control group (P＜0.05 or P＜0.01 ).In the hepatic tissues of n-hexane group, the levels of MDA and ADH significantly increased, the group (P＜0.05 or P＜0.01 ).In 2 GO groups, the gait scores and the staying time on the rotating rod were significantly improved, the levels of MDA and ADH significantly decreased, the activities of GSH-Px, T-AOCConclusion ADH could play an important role in the protective effects induced by garlic oil on the peripheral nerve injuries produced by n-hexane.%目的 研究大蒜油(garlic oil,GO)对正己烷(n-hexane)所致大鼠周围神经损伤的保护作用及其机制.方法 雄性Wistar大鼠40只,随机分为正常对照组、模型组、大蒜油低剂量组和高剂量组(n=10);正常对照组动物给予等体积生理盐水灌胃,模型组和大蒜油低、高剂量组大鼠分别给予2000mg/kg正己烷灌胃,大蒜油低、高剂量组大鼠于正己烷灌胃前1 h分别给予40和80mg/kg大蒜油灌胃,每周6次,持续10周.每周测步态评分和转棒指数,监测大鼠周围神经损伤情况.10周末,断头处死大鼠,测定肝组织中乙醇脱氢酶(ADH)、丙二醛(MDA)、还原型谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)、总抗氧化能力(T-AOC)、抑制羟基自由基能力.结果 与正常对照组相比,模型组大鼠步态评分明显升高,差异有统计学意义(P＜0.01),而转棒指数明显下降.肝脏中ADH活力[(3.64±0.73)U/mgpro]上升49.8%,肝脏中GSH-Px、T-AOC和抑制羟基自由基能力水平下降[分别为(182.0±41.7)、(1.10±0.27)、(162.1±26.1)U/mg pro],MDA含量[(1.89±0.42)nmol/mg pro]上升,与正常对照组[分别为(2.43±0.54)U/mgpro、(273.3±48.8)U/mg pro、(1.89±0.42)U/mg pro、(196.3±36.9)U/mg pro、(1.28±0.28)nmol/mg pro]的差异均有统计学意义(P＜0.05或P＜0.01).与模型组相比,大蒜油低、高剂量组大鼠步态评分明显降低,差异有统计学意义(P＜0.05),而转棒指数明显升高,差异均有统计学意义(P＜0.05或P＜0.01);与正常对照组相比,肝脏中ADH活力分别降低了25.3%和86.0%,GSH-Px、GSH、T-AOC及抑制羟基自由基能力水平明显上升,MDA含量明显下降,差异均有统计学意义(P＜0.05或P＜0.01).结论 大蒜油能够有效拮抗正己烷诱导所致的外周神经损伤,其机制可能与肝脏ADH活力降低有关.