重症肌无力中白介素4、15、18与端粒酶活性的相关性研究

摘要

Objective:To detect the serum levels of IL-4,IL-15 and IL-18 in myasthenia gravis( MG) and investigate the correlations between telomerase activity of CD4~+ T lymphocytes and these interleukins.Methods:The serum levels of IL-4,IL-15 and IL-18 were detected with ELISA and telomerase activity was detected by polymerase chain reaction enzyme-linked immonosorbent assay(PCR-ELISA) in 30 MG patients and 30 negative controls.Results:The levels of IL-4,IL-15,IL-18 and telomerase of CD4~+ T lymphocytes in MG were significantly higher than those in negative controls (P<0.01).There were positive correlations between the serum levels of IL-4,IL-15,IL-18 and telomerase activity of CD4~+ T cell in MG patients (P<0.01).Conclusion:IL-4,IL-15,IL-18 and telomerase activity of CD4~+ T lymphocytes are involved in the pathogenesis of MG;IL-4,IL-15 and IL-18 may upregulate the telomerase activity of CD4~+ T cells.%目的:探讨IL-4、IL-15、IL-18在重症肌无力(MG)中的表达及其与CD4~+T淋巴细胞端粒酶活性的相关关系.方法:应用ELISA及重复扩增-酶联免疫吸附实验(PCR-ELISA)检测30例MG患者(MG组)和30例健康对照者(NC组)血清中的IL-4、IL-15、IL-18水平和CD4~+T淋巴细胞端粒酶活性.结果:MG组血清IL-4、IL-15、IL-18水平及CD4~+T淋巴细胞端粒酶活性显著高于NC组,差异具有统计学意义(P<0.01); MG组CD4~+T淋巴细胞端粒酶活性与血清IL-4、IL-15、IL-18水平均存在正相关关系(P<0.01).结论:IL-4、IL-15、IL-18及CD4~+T淋巴细胞端粒酶均参与了MG的发病过程;MG中IL-4、IL-15及IL-18有可能通过各种途径直接或间接的上调CD4~+T淋巴细胞的端粒酶的活性.