TLR2激动剂Pam3CK逆转IL-10转染小鼠髓样树突状细胞免疫功能的研究

摘要

Objective :The efFects of TLR2 agonist on the immune f'unction of IL-10 transfected bone-marrow derived dendritic cell (mDC ) .Methods :The IL-10 plasmid was transfected to mouse mDC ,and TLR2 agonist Pam3CK was added to mDC for 48 hrs .The expression of MHC Ⅱ ,CD80 ,CD86 ,FasL in mDC was detected with flow cytometry ,and the production of the IL-6 and TNF-α was detennined by ELISA . Results :IL-10 suppressed the expression of CD80 ,CD86 and MHC Ⅱ ,decreased the production of IL-6 ,TNF-α ,and increased the expression of FasL on mDC .TLR2 agonist Pam3CK increased the expression of MHC Ⅱ ,CD80 and CD86 ,enhanced the production of IL-6 and TNF-α ,but inhibited of FasL expression by the IL-10 transfected mDC .Conclusion :TLR2 activation reverses IL-10 induced low-response of mDC .%目的:观察TLR2激活剂Pam3CK对IL-10转染小鼠髓样树突状细胞免疫功能影响.方法:转染IL-10至小鼠髓样树突状细胞(mDC),TLR2配体Pam3CK刺激48小时,利用流式细胞仪检测DC表面标志MHCⅡ、CD80、CD86及FasL等分子的表达; ELISA检测细胞产生IL-6、TNF-α.结果:IL-10抑制mDC表达CD80、CD86、MHC Ⅱ类分子,降低其分泌IL-6、TNF-α,促进其表达FasL,而TLR2激动剂刺激增加了IL-10转染DC表达MHC-Ⅱ类分子及CD80、CD86,促进其产生IL-6及TNF-α,抑制了FasL表达.结论:TLR2激动剂可逆转IL-10诱发的DC免疫应答低下.