3型肺炎球菌荚膜多糖结合疫苗的研制

摘要

目的：研制3型肺炎球菌荚膜多糖－CRM197结合疫苗。方法：将3型肺炎球菌的荚膜多糖与蛋白CRM197通过化学方法偶联结合，制备成多糖蛋白结合疫苗并使用该疫苗免疫幼小鼠。结果：经过实验条件的考察，发现在85℃，0．2mol／L 乙酸水解处理多糖1 h，活化度为10．0，投料比为20∶10时制得的疫苗，在小鼠体内产生了高滴度的抗体。结论：该实验条件下制备的结合疫苗保留了完好的抗原性，以本工艺条件制备3型肺炎球菌荚膜多糖蛋白结合疫苗是可行的。%Objective:To prepare Streptococcus pneumonia type 3 capsular polysaccharide conjugate vaccine.Methods:Strep-tococcus pneumonia type 3 capsular polysaccharide was covalently linked to protein CRM197 and its immunogenicity was evaluated in infant mice.Results:Through the preliminary research, we found that the polysaccharide was treated with 0.2 mol/L acetic acid at 85℃ for 1 h,activation grade reached to 10.0,and the radio of polysaccharide and protein was 20∶10,could induce infant mice to produce the high titers of antibodies.Conclusion:This result shows that conjugate vaccine prepared under this condition retained intact antigenicity.It is applicable to prepare Streptococcus pneumonia type 3 capsular polysaccharide conjugate vaccine by the process condi-tions.