两种免疫调节剂增强弓形虫W2b2a表位疫苗的小鼠保护作用

摘要

目的：探讨匹多莫德、沙利度胺增强弓形虫W2 b2 a表位疫苗刺激机体产生免疫应答和保护免疫的效果。方法：将匹多莫德、沙利度胺分别与弓形虫W2 b2 a表位疫苗混合肌注免疫小鼠，检测其细胞免疫和体液免疫，并观察其受到弓形虫攻击感染后的生存时间。结果：pcDNA3－W2b2a加匹多莫德组、pcDNA3－W2b2a加弗氏佐剂组、pcDNA3－W2b2a加沙利度胺组小鼠血清IFN－γ水平显著高于pcDNA3－W2b2a组（ P＜0．05）；pcDNA3－W2b2a加匹多莫德组、pcDNA3－W2b2a加弗氏佐剂组IgG抗体水平、CD4＋／CD8＋比值、T细胞增殖活性显著高于pcDNA3－W2b2a组和pcDNA3－W2b2a加沙利度胺组（ P＜0．05）；小鼠攻击试验表明，免疫组小鼠存活时间明显长于对照组（P＜0．05）。结论：匹多莫德、沙利度胺可增强弓形虫W2b2a表位疫苗免疫小鼠的保护作用。%Objective:To observe the effect of Pidotimod and Thalidomid to enhance the immune response and protective immunity induced by the epitope Vaccines From W2b2a of Toxoplasma gondii in mice.Methods:Mice were immunized intramuscularly with Pidotimod and pcDNA3-W2b2a,thalidomide and pcDNA3-W2b2a,respectively,then the immune response and the survival time of mouse attacked by Toxoplasma gondii were observed.Results:After the immunization,the level of IFN-γin sera of mice inculated with pcDNA3-W2b2a and Pidotimod,pcDNA3-W2b2a and Freund adjuvant,pcDNA3-W2b2a and Thalidomid were significantly higher than pcDNA3-W2b2a (all P<0.05).After the immunization,IgG,CD4+/CD8+T cell ratio ,proliferation of T cell induced by pcDNA3-W2b2a and Pidotimod,pcDNA3-W2b2a and Freund adjuvant were higher than pcDNA3-W2b2a,pcDNA3-W2b2a and thalidomid ( all P<0.05).After challenged with highly virulent tachyzoites,the mean survival time in immunized groups were significantly longer than control group (all P<0.05).Conclusion:Pidotimod ,Thalidomid adjuvant can increase protective immunity of epitope Vaccines From W2b2a of Toxoplasma gondii in mice.