来源未见原核(OPN)卵裂期胚胎培养至囊胚的临床应用分析

摘要

目的:分析来源OPN卵裂期胚胎培养至囊胚后行冷冻复苏周期的临床妊娠结局,评价来源OPN囊胚的临床应用价值.方法:回顾性分析2014年3月至2016年9月在安徽省妇幼保健院生殖医学中心接受辅助生殖助孕治疗中40个来源OPN的卵裂期胚胎培养至囊胚的冷冻复苏周期,分析其临床妊娠及分娩结局情况.结果:40个完全来源OPN的囊胚复苏周期移植的临床妊娠率为45.0％ (18/40),种植率为31.67％(19/60),随访截止时间至2016年9月,其中9例活产(5男4女),继续妊娠7例(8胎),2例流产(2胎),流产组织绒毛分析结果显示均异常.结论:患者行IVF-ET治疗后,可将来源OPN的卵裂期胚胎培养至囊胚给予冷冻,在没有来源2PN正常受精的卵裂期与囊胚时,患者可慎重选择来源OPN囊胚行复苏周期进行移植.%Objective:To analyze the clinical outcome of pregnancy of embryos at non-pronuclcus (OPN)cleavage stage which were cultured to blastocyst before cryopreservation,to evaluate the clinical value of OPN derived blastocyst.Methods:A retrospective analysis was conducted on the 40 embryos at OPN cleavage stage which were cultured to blastocyst before cryopreservation in the Anhui Provincial Maternal and Child Health Hospital from March 2014 to September 2016,to analyze the clinical pregnancy and childbirth outcomes.Results:The pregnancy rate was 45.0％ (18/40) and the implantation rate was 31.67％ (19/60).By the end of the follow-up of September 2016,there were 9 cases of live births (5 male and 4 female),7 cases of pregnancy (8 births) and 2 cases of abortion(2 births).Group analysis showed that abortion villi were abnormal.Conclusions:After receiving IVF-ET,embryos at non-pronuclcus(OPN) cleavage stage can be cultured to blastocyst to freeze in cleavage stage and be recovered for transplantation when there is no normal 2PN derived blastocyst.