CD31和CD105在卵巢上皮性肿瘤中的表达及其临床病理意义

摘要

目的 探讨CD31和CD105在卵巢上皮性肿瘤及正常卵巢组织中的表达情况及其与卵巢肿瘤生物学行为之间的关系,并比较同为肿瘤血管内皮标记物的CD31和CD105在标记微血管方面的差异.方法 收集天津市肿瘤医院临床、病理和预后资料完整的恶性卵巢上皮性肿瘤组织标本76例,病例标本采用免疫组化En Vision法检测CD31和CD105所标记的MVD数值,MVD计数参照Weidner方法进行量化分析,实验同时取20例交界性卵巢上皮性肿瘤、10例良性卵巢上皮性肿瘤和10例宫颈癌手术中切除的正常卵巢组织作为对照.结果 ①CD31蛋白在卵巢上皮性肿瘤的微血管和大血管上均有较强表达,在正常卵巢组织血管中亦有表达,恶性卵巢肿瘤中的MVD-CD31值(5.48±0.75)显著高于交界性卵巢肿瘤(2.24±0.61)、良性卵巢肿瘤(2.24±0.41)及正常卵巢组织(1.20±0.37)(P＜0.01)；在卵巢癌中,MVD-CD31值仅与有无淋巴结转移及组织学分级之间的差异有统计学意义(P＜0.05),而与年龄、病理类型、肿瘤大小、腹水、有无远处转移无关(P＞0.05).②CD105蛋白在卵巢肿瘤微血管中有表达,在正常卵巢组织中呈微弱表达或无表达,恶性卵巢肿瘤中的MVD-CD105值(4.07±2.11)显著高于交界性卵巢肿瘤(2.08±0.30)、良性卵巢肿瘤(1.92±1.15)及正常卵巢组织(0.68±0.39)(P＜0.05或P＜0.01)；在卵巢癌中MVD-CD105值与组织学分级、有无腹水、有无远处转移、有无淋巴结转移有关(P＜0.05),而与年龄、病理类型、肿瘤大小等因素无关(P＞0.05).③恶性肿瘤组织中的M VD-CD31值显著高于MVD-CD105值(P＜0.05).结论 在标染卵巢癌方面,CD105比CD31有明显优越性,CD105的表达与卵巢癌的生物学行为密切相关,MVD-CD105值的检测可更准确的确定肿瘤的临床分期、指导治疗及判断预后.%Objective To investigate the expression of CD31 and CD105 in epithelial ovarian tumor and normal ovarian tissues, then compare the differences between CD31 and CD105 in marking the endothelial cells. Methods 76 ovarian cancer specimens with complete clinical and prognostic data were collected in Tianjin Medical University Cancer Institute and Hospital. We used immunohistochemistry method to detect CD31 and CD105, and Weidner method to count the tumor's MVD marked by CD31 and CD105. We also collected 20 borderline ovarian tumor tissues, 10 benign ovarian tumor tissues and 10 normal ovarian tissues as control groups. Results ①CD31 was expressed in both microvessels and great vessels in ovarian tumors and normal ovarian tissues. MVD-CD31 value in ovarian cancer was significant higher than in control groups. In ovarian cancer, MVD-CD31 value was closely related to lymph node metastasis and histological grades(P<0. 05), but not related to age, histological types, tumor size, ascites and metastasis (P > 0. 05). ② CD105 was only expressed in microvessels of ovarian tumor, but not expressed in normal ovarian tissues. MVD-CD105 value in ovarian cancer was significant higher than in control groups. In ovarian cancer, MVD-CD105 value was closely related to histological grades, ascites, metastasis and lymph node metastasis (P<3. 05), but not related to age, histological types and tumor-size(P>0. 05). ③ In ovarian cancer, MVD-CD31 value was significant higher than MVD-CD105 value(P<0. 05). Conclusion CD105 is better than CD31 in staining ovarian cancer, and MVD-CD105 value is closely related to the biological behavior of ovarian cancer. Thus detection of CD105 is recommended to precisely assess tumor stage, to direct cancer therapy and to predict prognosis.