蛋白激酶PRKX对人肝癌细胞粘附和迁移能力的影响

摘要

Objective To investigate the possible effect of PRKX on the adhesion and migration of the human hepatocellular carcinoma cell line SMMC-7721. Methods The SMMC-7721 cells were transfected with pDONR223-PRKX with Lipofectamine 2000. The expression of PRKX protein in SMMC-7721 cells was i-dentified by Western blot. The adhesion of SMMC-7721 cells was studied by cell-matrix adhesion assay. Cell migration assay was used to determine the changes in migration of SMMC-7721 cells. Results The expression of PRKX protein in SMMC-7721 cells was increased after pDONR223-PRKX transfection. The adhesion and migration of SMMC-7721 cells were enhanced in the transfected group compared with those of control group. Conclusion PRKX increases the adhesion and migration ability of human hepatocellular carcinoma cell line SMMC-7721.%目的 观察蛋白激酶PRKX对人肝癌细胞SMMC-7721粘附和迁移能力的影响.方法 采用脂质体转染的方法,将PRKX表达质粒转染到SMMC-7721细胞中,蛋白印迹方法鉴定转染前后PRKX蛋白的表达.细胞-基质粘附实验测定对照组和PRKX转染组SMMC-7721细胞的粘附能力.细胞迁移实验测定对照组和PRKX转染组SMMC-7721细胞的迁移能力.结果 SMMC-7721细胞转染组PRKX蛋白的表达增加,SMMC-7721细胞转染组的粘附能力和迁移能力均较对照组增加.结论 PRKX可增加人肝癌细胞SMMC-7721的粘附和迁移能力.