ROCK特异性抑制剂Y27632对缺氧后少突胶质前体细胞分化的影响

摘要

Objective To study the effect of ROCK pathway inhibition by Y27632,a special ROCK inhibi-tor,on OPC differentiation following hypoxia ischemia in vitro.Methods The cultivated OPC from rat cerebral cor-tex were randomly divided into 4 groups:control,control+Y27632,OGD,and OGD+Y27632.The expression of MBP was evaluated in OPC after 2h exposure to oxygen-glucose deprivation (OGD)by immunofluorescence tech-nique and Western blot assay.Results Compared with that of control groups,the expression of MBP was in-creased in OGD groups (P<0.01).Meanwhile,the expression of MBP was up-regulated in the OGD+Y27632 group compared with that of the OGD group (P<0.01).Conclusion OGD promotes the differenti-ation of OPC.ROCK inhibition by Y27632 can remarkably promote the differentiation of OPC after OGD. It is suggested that the ROCK signaling pathway plays an important role in the regulation of hypoxia-in-duced differentiation of OPCs.%目的：观察ROCK特异性抑制剂Y27632对缺氧损伤(Oxygen-glucose deprivation,OGD)后少突胶质前体细胞分化的影响。方法培养 SD大鼠大脑皮层少突胶质前体细胞,实验分为对照组、对照+Y27632组、OGD组、OGD+Y27632组四组；对细胞进行 OGD处理2 h,取4 d后时间点,进行免疫荧光组化染色和 Western blot 实验,检测细胞 A2B5、NG2、O4及 MBP蛋白表达情况。结果与对照组相比,OGD组表达少突胶质细胞特异性蛋白 MBP 量明显增多(P＜0.01)；OGD+Y27632组比单纯 OGD组表达少突胶质细胞特异性蛋白 MBP 的量显著增加(P＜0.01)。结论 OGD损伤可促进 OPCs 的分化,Y27632特异性抑制ROCK可以进一步促进OGD损伤后OPCs的分化,提示ROCK信号通路在缺氧诱导OPCs分化的过程中有重要的调控作用。