内分泌腺来源的血管内皮生长因子对胰腺癌细胞增殖和凋亡的影响

摘要

目的 探索内分泌腺来源的血管内皮生长因子(EG-VEGF)对胰腺癌细胞增殖和凋亡的影响以及对PI3K/AKT信号通路的调控作用.方法 胰腺癌组织及癌旁组织取自2015年1月至2016年12月宁夏医科大学总医院经临床及病理学检查确诊为胰腺癌的患者手术标本30例.利用聚合酶链式反应(PCR)检测EG-VEGF和其受体——血管内皮生长因子受体2(VEGFR-2)在胰腺癌组织、癌旁组织中的表达.通过MTT方法检测EG-VEGF处理对胰腺癌细胞增殖的影响;通过流式细胞技术检测EG-VEGF处理对胰腺癌细胞凋亡的影响;应用蛋白印迹检测EG-VEGF处理后PI3K/AKT信号通路中相关蛋白表达变化.结果 EG-VEGF和VEGFR-2在胰腺癌组织中表达水平分别为(3.08±0.30)和(2.17±0.16),显著高于癌旁组织(分别为1.55±0.73、0.54±0.34,均P＜0.05).MTT实验和流式细胞术检测表明,EG-VEGF能够促进胰腺癌细胞增殖,同时能够抑制细胞凋亡.蛋白印记(western blot)检测表明,EG-VEGF促进AKT磷酸化而激活PI3K/AKT信号通路.结论 EG-VEGF在胰腺癌组织中高表达,并且能够激活PI3K/AKT信号通路,从而促进胰腺肿瘤细胞增殖和抑制其凋亡.%Objective To investigate the effect of endocrine gland-derived vascular endothelial growth factor(EG-VEGF) on the proliferation and apoptosis of pancreatic cancer cells and on the regulation of PI3K/AKT signaling pathway.Methords The expression of EG-VEGF and its receptor-VEGFR-2 in pancreatic cancer tissues and adjacent normal tissues was detected by PCR.The effect of EG-VEGF on proliferation of human pancreatic cancer cells was detected by MTT assay.The effect of EG-VEGF on the apoptosis of human pancreatic cancer cells was detected by flow cytometry.Western lot was used to detect the changes of related proteins expression in PI3K/AKT signaling pathway after EG-VEGF treatment.Results The expression (3.08±0.30,2.17±0.16 respectively) of EG-VEGF and VEGFR-2 in pancreatic cancer tissues was significantly higher than those (1.55±0.73,0.54±0.34 respectively) in adjacent tissues (both P＜ 0.05).MTT and flow cytometry data showed that EG-VEGF could promote the proliferation of pancreatic cancer cells and inhibit cell apoptosis.Western blot showed that EG-VEGF promoted AKT phosphorylation and activated PI3K/AKT signaling pathway.Conclusion EG-VEGF is highly expressed in pancreatic cancer tissue,and can activate PI3K/AKT signaling pathway to promote cell proliferation and inhibit cell apoptosis.