腹腔注射胰腺炎相关性腹水与穿孔性腹膜炎腹水诱导大鼠急性肺损伤的对比研究

摘要

Objective To establish a rat model of acute lung injury (ALI) induced by intraperitoneal injection of pancreatitis associated ascitic fluids (PPAF) and perforative peritonitis ascitic fluids (PAAF).A secondary objective is to study the non-specificity of acute lung injury induced by PAAF.Methods Acute necrotizing pancreatitis (ANP) model and perforative peritonitis (PP) model were established in 120 rats,from which the PAAF and PPAF were collected.Forty-eight rats were randomly divided into three groups:normal saline (NS) group,PAAF group,and PPAF group.Within each group,they were randomly sacrificed at 7h and 12h of surgery.The pathological severity of the lung injury,wet/dry ratio,MPO (myeloperoxidase) in lung,and apoptosis rate of pneumocytes were evaluated and analyzed.Results Lung injury,wet/dry ratio,MPO in lungs,and cell apoptosis were significantly higher in the PAAF group and PPAF group than in the NS group (P＜0.01).However,there was no significant difference between PAAF group and PPAF group (P＞0.05).Conclusions Both PAAF and PPAF can induce acute lung injury in rats by intraperitoneal injection.However,the acute hung injury induced by PAAF has limited specificity.%目的 通过腹腔注射胰腺炎相关性腹水(pancreatitis associated ascetic fluids,PAAF)和上消化道穿孔性腹膜炎腹水(perforative peritonitis ascitic fluids,PPAF)诱导急性肺损伤(acute lung injury,ALI),探讨腹腔注射PAAF诱导大鼠ALI的非特异性.方法 120只SD大鼠建立急性坏死性胰腺炎模型和消化道穿孔模型,收集PAAF和PPAF；48只大鼠随机分为生理盐水对照组(n=16),PAAF组(n=16)和PPAF组(n=16).各组大鼠随机分为7h、12h亚组.检测肺组织病理损害评分、肺组织湿/干比、肺组织髓过氧化物酶和肺细胞凋亡检测.结果 PAAF组和PPAF组各时间点肺组织髓过氧化物酶、肺组织病理损害评分、湿/干比和细胞凋亡率均高于对照组(P＜0.01),而PAAF组和PPAF组各指标间差异无统计学意义.结论 腹腔注射PAAF和PPAF均可诱导大鼠ALI,腹腔注射PAAF诱导大鼠ALI具有非特异性.