雌激素受体α启动子区甲基化与冠状动脉粥样硬化的关系

摘要

目的 探讨雌激素受体(ER)-α基因甲基化状态在冠状动脉粥样硬化发生中的作用.方法 采用甲基化特异性PCR(MSP)技术检测40例冠心病患者外周血ER-α基因启动子区甲基化状态,以20例健康成人为对照组.采用Real-time RT-PCR检测ER-α基因的mRNA表达水平.结果 实验组ER-α基因启动子区甲基化阳性率显著高于对照组分别为77.5%和55%;对照组人群ER-α基因的mRNA表达水平是实验组的2.57倍.结论 在冠状动脉粥样硬化患者外周血中存在ER-α基因的高甲基化和低表达状态,可能参与其发生发展过程.%Objective To discuss the relationship between methylation of ER-α gene CpG island and coronary atherosclerosis.Methods 60 cases including 40 coronary atherosclerosis patients and 20 healthy control subjects.Methylation-specific polymerase chain reaction was used to detect promoter methylation status of the ER-t and the mRNA expression were investigated by real time RT-PCR.Results The methylation of ER-α gene was increased in coronary atherosclerotic patients compared to healthy people, while mRNA expression of ER-α in controlled group was 2.57 times as much as that of coronary atherosclerotic patients.Conclusions The aberrant methylation and low expression of ER-α may contribute to the development of coronary atherosclerosis.