黄连乙醇提取物对生理及链脲佐菌素致病理状态下小鼠抑郁和焦虑行为的影响

摘要

目的 探讨黄连55%乙醇提取物(CCEE)对生理状态及链脲佐菌素(STZ)致病理状态下小鼠抑郁和焦虑行为的干预作用.方法 CCEE分别以低剂量(50 mg/kg)和高剂量(200 mg/kg)连续灌胃(ig)给予生理状态及STZ病理状态下的小鼠21 d, 1次/d,末次给药后1 h,进行开场实验(OFT)、明暗箱实验(LDT)、高架十字迷宫实验(EPM)、悬尾试验(TST)和强迫游泳实验(FST)行为学测试.结果 CCEE高剂量(200 mg/kg)对生理状态及STZ病理状态下小鼠在OFT测试中的运动时间均显著升高、在TST和FST的不动时间均显著降低、在EPM的开臂进入次数百分比( OE% )均显著升高;对STZ病理状态下小鼠在OFT测试中的站立次数及在EPM的开臂滞留时间百分比(OT% )均显著升高,而对其在生理状态下均无显著影响;CCEE低剂量(50 mg/kg)显著降低STZ病理状态下小鼠在TST中的不动时间,而对在生理状态下无显著影响.结论 CCEE高剂量对生理状态及STZ病理状态下小鼠抑郁和焦虑行为均有改善作用,而CCEE低剂量仅对STZ病理状态下小鼠的抑郁和焦虑行为具有改善作用;一定程度上, CCEE对STZ病理状态下的治疗作用较对其在生理状态下的作用更为敏感.%Objective To study the effects of Coptis chinensis Franch. 55% ethanol extract (CCEE) on depression and anxiety be-havior in mice under morphological state and streptozotocin (STZ) pathological state. Methods CCEE was given oral administration at low dose (50 mg/kg) or high dose (200 mg/kg) for consecutive 21 days, once a day, to mice under morphological state and STZ pathological state. At 1 h after the last administration, open field test (OFT), light-dark test (LDT), behavioral tests including elevated plus maze (EPM), tail suspention test (TST) and forced swimming test (FST) were performed. Results CCEE high dose (CCEE-H, 200 mg/kg) significantly elevated the exercise time in the OFT and the OE% in the EPM test, and reduced the immobility time in the TST and FST in mice under both morphological state and STZ pathological state. CCEE low dose (CCEE-L, 50 mg/kg) significantly increased the standing times in the OFT and the OT% in the EPM under the STZ pathological state instead of the morphological state of mice. Besides, CCEE-L significantly decreased the immobility in the TST only under the STZ pathological state instead of the morphological state of mice. Conclu-sions CCEE-H could improve depression and anxiety behavior in mice under both morphological state and STZ pathological state, while CCEE-L only improve the depression and anxiety behavior under the STZ pathological state instead of the morphological state of mice. These findings suggest that, to a certain extent, the therapeutic effect of CCEE on STZ in the pathophysiological state is more sensitive than that in the physiological state.