载脂蛋白基因与空间记忆力及胆固醇代谢的相关性分析

摘要

Objective To investigate the role of apolipoprotein E (apoE4) gene in Alzheimer's disease.Methods The ApoE4 inbred transgenic mice were generated via microinjection.Southern blot and Northern blot hybridization were used to indentify the integration and expression of the transgene,respectively.Enzymatic method was used to measure serum cholesterol.Y-maze was used to test the memory function.Results The serum cholesterol level in the 12- month transgenic mice[(3.02±0.14) mmol/L]was higher than that in control mice[(2.11±0.01) mmol/L,P ＜ 0.01].The memory function was decreased in transgenic mice[(0.82±0.05)％]in comparison with control mice[(0.98±0.03)％, P ＜ 0.05].Conclusion The results suggest that the apoE4 gene may be related to the higher serum cholesterol level,and the overexpression of apoE4 gene in inbred transgenic mice impairs primitive working memory capacity.%目的 研究载脂蛋白E(apoE)基因在早老痴呆疾病中的作用.方法 选取C57BL/6J鼠,通过显微注射法建立人突变apoE4近交系转基因鼠,PCR初筛,再将首建鼠与正常C57BL/6J鼠繁育,将获得的6只转基因鼠作为转基因组,经Southern杂交鉴定,同时出生的结果为阴性的6只小鼠作为对照组,用酶法测定2组小鼠2、9和12月龄血清TC水平,Y迷宫测定2组小鼠9和12月龄空间记忆能力.结果 2组小鼠2和9月龄时,血清 TC水平差异无统计学意义(P＞0.05),12月龄时转基因组鼠血清TC水平(3.02±0.14)mmol/L,较同龄对照组鼠明显升高(2.11±0.01)mmol/L,差异有统计学意义(P＜0.01).转基因组鼠9和12月龄时空间记忆力[(0.82±0.49)%,(0.82±0.05)%」较对照组鼠[(0.99±0.05)%,(0.98±0.03)%]明显下降,差异有统计学意义(P＜0.05).结论 apoE4基因与TC代谢可能具有相关性,同时apoE4基因过渡表达损伤了转基因鼠的记忆能力.