胸苷激酶1和生存素在甲状腺癌及腺瘤组织的表达及其临床意义

摘要

目的 探讨甲状腺癌及腺瘤组织中胸苷激酶1(TK1)和生存素(Survivin)的表达及临床意义.方法 收集行手术治疗的107例甲状腺肿瘤患者的组织标本,包括24例甲状腺瘤、83例甲状腺癌组织,另取15例正常甲状腺组织作为对照组;采用免疫组织化学法检测各组织中TK1和Survivin的表达水平.结果 TK1在正常甲状腺组织、甲状腺瘤组织和甲状腺癌组织中的表达率分别为6.67％ (1/15)、41.67％ (10/24)和67.47％(56/83),两两之间比较差异有统计学意义(分别为t=5.244、19.301、5.584,P＜0.05),TK1表达水平与甲状腺癌美国肿瘤联合委员会(AJCC)分期、颈部淋巴结转移、包膜浸润明显相关(分别为t =4.329、4.506、4.747,P＜0.05);Survivin在正常甲状腺组织、甲状腺瘤组织和甲状腺癌组织中的表达率分别为6.67％(1/15)、37.50％(9/24)和61.45％(51/83),两两之间比较差异有统计学意义(分别为t=4.334、15.306、4.603,P＜0.05),Survivin表达水平与甲状腺癌AJCC分期、组织分化、颈部淋巴结转移、包膜浸润明显相关(分别为t=6.295、8.967、6.116、6.005,P＜0.05).结论 TK1和Survivin在甲状腺癌组织中高表达、是甲状腺癌的不良预后因素;检测TK1和Survivin有助于判断甲状腺癌浸润转移潜能.%Objective To investigate the expression of thymidine kinase 1 (TK1) and Survivin in thyroid carcinoma and thyroid benign tumor,and to analyze the clinical significance.Methods The tissue samples of 107 patients with thyroid tumor who underwent surgical resection were collected,including 24 cases of thyroid benign tumor tissues,83 cases of thyroid carcinoma tissues,and 15 cases of thyroid normal tissues selected as the control group;Immunohistochemical method staining for the paraffin sections (SP method)were used to assess the expression of TK1 and Survivin expression in thyroid carcinoma tissues,thyroid benign tumor tissues and thyroid normal tissues.Results The expression rate of TK1 in thyroid normal tissues,thyroid benign tumor tissues and thyroid carcinoma tissues were 6.67％ (1/15),41.67％ (10/24) and 67.47％ (56/83) with significant difference (Respectively,t =5.244,19.301,5.584,P ＜ 0.05),the expressions of TK1 were related thyroid carcinoma to American Joint Committee on Cancer (AJCC) clinical stage,lymph node metastasis and the degree of infiltration (Respectively,t =4.329,4.506,4.747,P ＜0.05);the expression rate of Survivin in thyroid normal tissues,thyroid benign tumor tissues and thyroid carcinoma tissues were 6.67％ (1/15),37.50％ (9/24) and 61.45％ (51/83) with significant difference (Respectively,t =4.334,15.306,4.603,P ＜ 0.05),the expressions of Survivin were related thyroid carcinoma to AJCC clinical stage,differentiation degree,lymph node metastasis and the degree of infiltration (Respectively,t =6.295,8.967,6.116,6.005,P ＜ 0.05).Conclusion TK1 and Survivin are highly expressed in thyroid carcinoma tissues,TK1 and Survivin are prognostic factor for thyroid carcinoma patients;Detecting the expressive levels of TK1 and Survivin in thyroid carcinoma have certain reference value for judging invasion and metastasis potential of patients of thyroid carcinoma.