生存素、Livin和zeste同源物-2在膀胱癌组织中的表达

摘要

Objective To investigate the expression of survivin,livin and enhancer of zeste homolog-2 (EZH-2) in bladder cancer tissues and their correlation with bladder cancer.Methods Eighty-three patients pathological diagnosed as bladder cancer were randomly selected as the observation group.Fifty patients with bladder benign lesions served as the control group.The bladder cancer tissues from observation group and the normal bladder mucosa sanples from control group were obtained detection of survivin,livin and EZH-2.The relationship between the expression of survivin,livin and EZH-2 with the clinical data was analyzed.All patients were subjected to 5-year outpatient follow-up.Results In the bladder cancer tissues,the positive expression rate of survivin,livin and EZH-2 was 74.70％,69.88％ and 83.13％ respectively,significantly higher than in normal bladder tissues correspondingly (P ＜ 0.05).The positive expression of survivin and livin was not related with sex,TNM stage,pathological grading and the number of tumor (P ＞ 0.05),but related to tissue origin and tumor recurrence (P ＜ 0.05).The positive expression of EZH-2 had no correlation with the number of tumor and gender (P ＞ 0.05),but related with tissue origin,TNM staging,pathological grading and recurrence of the tumor (P ＜ 0.05).There was a positive correlation between survivin and livin (r =0.744,P ＜ 0.01),or between survivin and EZH-2 (r =0.717,P ＜ 0.05),or between livin and EZH-2 (r =0.683,P ＜ 0.05).Conclusion The expression of survivin,livin and EZH-2 in bladder cancer tissues was significantly higher than that in normal bladder tissue,and related to the occurrence and development of tumor.The detection of the positive expression rate of survivin,livin and EZH-2 can evaluate the prognosis.%目的 探讨膀胱癌组织中凋亡抑制基因生存素(Survivin)、Livin和zeste同源物-2(EZH-2)基因的表达及其相关性.方法 随机选取经病理检查明确诊断为膀胱癌的患者83例作为观察组,另选膀胱良性病变患者50例作为对照组,取观察组患者的膀胱癌组织和对照组患者正常的膀胱黏膜作为检测标本,利用免疫组织化学方法对标本中凋亡抑制蛋白Survivin、Livin和EZH-2蛋白染色,观察凋亡抑制基因Survivin、Livin和EZH-2基因的表达,并分析与临床资料的关系.对所有观察组患者进行为期5年的门诊随访.结果 膀胱癌组织中,Survivin、Livin和EZH-2的阳性表达率分别为74.70％、69.88％和83.13％,3种蛋白的阳性表达率均显著高于正常膀胱组织(P＜0.05);Survivin和Livin的阳性表达与性别、TNM分期、病理分级和肿瘤数目无明显相关(P＞0.05),而与组织来源和肿瘤复发明显相关(P＜0.05);EZH-2的阳性表达与性别、肿瘤数目无明显相关(P＞0.05),而与组织来源、TNM分期、病理分级和肿瘤复发明显相关(P＜0.05);Survivin和Livin的表达呈正相关(r=0.744,P＜0.01),Survivin和EZH-2的表达呈正相关(r=0.717,P＜0.05),Livin和EZH-2的表达呈正相关(r=0.683,P＜0.05).结论 Survivin、Livin和EZH-2在膀胱癌组织中的表达显著高于正常膀胱组织,且与肿瘤的发生发展有关,其阳性率的检测有助于判断其预后.