缺氧诱导因子－1和果蝇 zeste 基因增强子人类同源物蛋白在结直肠癌中的表达及临床意义

摘要

目的：研究缺氧诱导因子－1（hypoxia inducible factor，HIF－1）和果蝇zeste基因增强子人类同源物（enhancer of zeste homolog 2，EZH2）蛋白在结直肠癌组织及正常组织的表达，并探讨与临床病理因素的关系及两者表达的相关性。方法：应用免疫组织化学法分析检测HIF－1和EZH2蛋白的表达情况并同时分析两者表达的相关性。结果：HIF－1和EZH2蛋白在结直肠癌组织中高表达，在正常组织中低表达或无表达，差异有统计学意义（ P ＜0．05）。两者的表达与性别、年龄、是否吸烟、分期及病理类型未见相关（ P ＞0．05），与淋巴结转移及浸润密切相关（ P ＜0．05）。两者间在组织中表达呈正相关（ r ＝0．413， P ＝0．018）。结论：HIF－1和EZH2蛋白在结直肠癌的发生发展中发挥着重要作用，且有可能作为临床判断预后的标志物。%Objective:To study the expressions of HIF -1 and EZH2 protein in colorectal carcinoma and normal issues and to explore their re-lationship with clinical pathological features and their expression correlation .Methods:The immunohistochemistry method was applied to determine the expression of HIF-1 and EZH2 protein,and their correlation was statistically analyzed .Results:There was significant difference with high ex-pression of HIF-1 and EZH2 protein in human colorectal carcinoma tissues and low or no expression in normal tissues ( P <0.05).Their expres-sions had no relationship with gender, age, smoking, clinical stage and pathologic type ( P >0.05),but were closely associated with lymphatic vessel invasion and metastasis ( P <0.05).Their expressions in the tissues were positively correlated( r =0.413, P =0.018).Conclusion:The expressions of HIF-1 and EZH2 protein may be closely related to the occurrence and development of colorectal carcinoma and can be used as po-tential prognostic marker.