背景 巯基化合物包括谷胱甘肽和其他抗氧化活性物质,还原型谷胱甘肽分布在视网膜不同的细胞层中,是重要的水溶性抗氧化物质.糖尿病可致视网膜的抗氧化反应受到明显影响,测定糖尿病大鼠视网膜中巯基化合物的质量分数可以用来探讨其抗氧化治疗的作用.目的 探讨肌肽、阿司匹林滴眼液对糖尿病大鼠视网膜中巯基化合物的影响.方法 102只清洁级SD大鼠,随机抽取12只为正常对照组,其余90只制作糖尿病模型.造模成功的大鼠76只用随机数字表法随机分为模型组、肌肽组、阿司匹林组及联合组,每组19只.各组分别于造模成功后给予缓冲液、质量分数1%肌肽、质量分数0.05%阿司匹林、1%肌肽+0.05%阿司匹林点双眼,每只眼点2滴,每日2次,共8周;正常对照组不给药.给药第8周时处死所有大鼠,取各组大鼠视网膜制成组织匀浆,根据DTNB的原理,在25℃、412 nm的吸收峰处用谷胱甘肽测定试剂盒通过比色法测定视网膜中巯基化合物的质量分数.结果 与正常对照组相比,其他各组大鼠的体质量均明显减轻,联合组大鼠的体质量明显高于除正常对照组以外的其他组大鼠,但差异无统计学意义(P＞0.01).与正常对照组相比,其他各组大鼠的血糖水平均明显升高.模型组视网膜中巯基化合物的质量分数为(190.35±46.62)mg/gprot,低于正常对照组的(202.77±53.49)mg/gprot;肌肽组为(378.40±165.06)mg/gprot,阿司匹林组为(351.88±211.62)mg/gprot,均高于正常对照组,差异均有统计学意义(P＜0.05).联合组巯基化合物的质量分数为(166.972±50.287)mg/gprot,低于正常对照组和模型组,但差异均无统计学意义(P＞0.05).结论 肌肽、阿司匹林可稳定糖尿病大鼠视网膜组织中还原型巯基化合物的表达,具有明显的抗氧化损伤保护作用,但二者联合使用并无明显的协同作用.%Background The reduced glutathione(GSH) is a water soluble antioxidant in retina and it is an important component of thiol.Diabetes induce the oxidative response of retina.It is very important to detect the content of thiol in retina for the evaluation of its antioxidant role.Objective This study was to investigate the effect of carnosine,aspirin and a combination of carnosine and aspirin eye drops on the change of the thiol contents in the retina of the diabetic rats. Methods Diabetic models were induced in 76 clean male Sprague-Dawley(SD) rats by intraperitoneal injection of streptozotocin(65 mg/kg).The 0.02 mi sodium citrate buffer was injected in 12 control rats.1%camosine eye drops or 0.05%aspirin eye drops were topically administered respectively in carnosine group (19 rats)or aspirin group(19 rats)two times per day for a period of 8 weeks,and the combination of 1%carnosine eye drops and 0.05%aspirin eye drops was used in the combination group at the same way(19 rats).At the end of 8 weeks.The animMs were sacrificed and the eyeballs were enucleated for the detect of thiols in the retina by colorimetry.Results The body weight of SD rats was significantly reduced and the blood glucose level was evidently arose in model group,camosine group and aspirin group in comparison with control group(P＜0.05),but those of combination group compared with control group was insignificantly changed(P＞0.05).The level of thiol in retina was(1 90.35+46.62)ms/gprot in model group,showing a considerable decrease in comparison with control (202.77+53.49 ms/gprot)(P＜0.05).However,the thiol level rose to(378.40±165.06)mg/gprot in camosine group and(351.88±211.62)mg/gprot in aspirin group,showing a statistically significant difference in comparison with control group(P＜0.05).The content of thiol in combination group compared with control group Was insignificant changed(166.972±50.287 mg/gprot us 202.77±53.49 mg/gprot).Curiously the levels in the combination group were not different from the normal control group. Conclusion The carnosine and aspirin eye drops may protect the retina against the oxidative damage caused by diabetes.
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