Objective To detect HBV-LP and HBV DNA of the patients with hepatitis B, and to study the sensitive and specificity of HBV-LP detecting and its evaluate on for clinical application. Methods The ELISA was used for HBV-LP detecting and RT-PCR for HBV DNA detecting. Results The sensitive and specificity of HBV-LP and HBV DNA were 64.89% ,99.68% ,60.63% and 100% respectively(P > 0.05);+LR, -LR were 202.78,60.63, 0.3522 and 0.3937, and there were significance between +LR of the detecting (P<0.005 ). Conclusion The sensitive and specificity of HBV-LP and HBV DNA detecting are considerable, +LRof HBV-LP are higher comparing HBV DNA. The HBV-LP is better serology index for detecting replication of HBV DNA.%目的 检测乙肝患者的HBV-LP及HBV DNA,探讨HBV-LP的敏感性和特异性及其临床应用价值.方法 HBV-LP检测采用ELISA法,HBV DNA检测采用RT-PCR法.结果 HBV-LP检测及HBV DNA检测的敏感性分别为64.89%、99.68%和60.63%、100%(P>0.05);两检测的+LR和-LR分别为202.78、60.63和0.3522、0.3937,+LR之间差异有统计学意义(P<0.005).结论 HBV-LP检测有与HBV DNA检测相近的敏感性及特异性,阳性似然比更高于HBV DNA检测,为较好的检测HBV DNA复制的血清学指标.
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