HIV/AIDS患者体内Th17及Th1应答失衡

摘要

目的 探讨HIV/AIDS患者体内Th17、Th1应答情况及Th17与Th1应答之间的相互关系.方法 选取38例符合诊断标准的慢性HIV感染患者,根据抗病毒治疗与否,将其分为2组:治疗前16例,治疗后22例,同时选取24例健康志愿者为对照.采用全血胞内细胞因子染色方法,使用BD FACSCanto流式细胞仪检测各项指标,FACSDiva软件分析CD4~+IL-17~+T细胞及CD4~+IFN-γ~+T细胞表达情况,将结果进行统计学分析并比较各组之间的差异.结果 未治疗患者CIM~+IL-17~+T细胞表达显著低于健康对照(1.14±0.79)%vs(3.98±1.14)%,P=0.000,经抗毒治疗后明显升高(2.22±1.00)%,P=0.001;而CD4~+IFN-γ~+T细胞则在治疗前后没有显著变化(34.35±24.38% vs42.10+15.57%),与健康人比较亦差异无统计学意义P=0.383;进一步相关性分析表明CD4~+IL-17~+T细胞与CD4~+T细胞计数呈正相关(R=0.345,P=0.034),而CD4~+IFN-γ~+T细胞则与CD4~+T细胞计数没有明显相关性(R=-0.247,P=0.136).结论 人体感染HIV病毒以后,机体出现Th17应答显著下调,Th17/Th1平衡紊乱,Th17免疫应答可能在HIV感染致病机制中起着重要作用.%Objective To evaluate the Th17/Th1 response in HIV infected patients and the mutual relationship between the response of Th17 and Th1. Methods 38 chronic HIV infected patients as well as 24 healthy volunteers were performed in this study. The patients were divided into two groups, one group before treatment, the other after therapy. The whole blood intracellular cytokine staining was used, samples detected by BD FACSCanto, after that, the expression of CD4~+IL-17~+T cell and CD4~+IFN-γ~+T cell were analyzed by FACSDiva software and lastly compared the differences among different groups. Results The expression of CD4~+IL-17~+T cell in naive-therapy patients were significantly lower than that of the healthy controls(1.14±0.7)9% vs (3.98±1.14) % , P=0.000, but increased remarkably after HARRT(highly antiretroviral treatment) (2.22±1.00)% ,P=0.001;however there were no significant differences in the expression of CD4~+IFN-γ~+T cell before and after therapy(34.35±24.38)% vs (42.10±15.57% ) ,also with the healthy control(P=0.383 ). The frequency of CD4~+IL-17~+T cell was positively correlated with CD4~+T counts(R=0.345,P=0.034), but no significant correlations was observed between the expression of CIM~+IFN-γ~+T cell and CD4~+T counts (R=-0.247, P=0.136 ). Conclusion The infection of HIV virus down-regulated Th17 immune response and disturbed the balances between Th17 and Th1 evidently in human. Th17 response may play an important role in the pathogenesis of HIV infection.