乙酰半胱氨酸镁对大鼠肝硬化门静脉高压的保护作用

摘要

Objective To study the effects of acetylcysteine magnesium on the vasoactive substances and hepatic fibrosis indexes in liver cirrhosis and portal hypertension of rats.Methods The rat liver cirrhosis model was made with 12 μg/kg dimethylnitrosamines.Then acetylcysteine magnesium was injected respectively with 25,50,and 100 mg· kg-1 dose daily into abdominal cavity.After 8 weeks treatment,pathological section,TGF-β1,NO,TNOS and iNOS of hepatic tissue were detected to assess the effect of acetylcysteine magnesium against cirrhosis portal hypertension.Results After the DMNA modeling was completed,the HE and Sweet reticulocyte staining of liver pathological section showed that cirrhosis of the liver was in the Ⅲ-Ⅳ phase,the infiltration of lymphocytes and formation of pseudolobuli in liver were alleviated in three acetylcysteine magnesium treatment groups (low,medium,and high dose),and the degree of liver fiber sclerosis in three groups was significantly lower than control group.Compared with control group,TGF-β1,NO,TNOS and iNOS were significantly reduced in all treatment groups(P ＜0.05).Conclusion Acetylcysteine magnesium is probably a distinctive antioxidant which can remove various free radical in body and modulate ligand-dependent signal transduction and the growth of cell.It also have protection in the liver cirrhosis and portal hypertension of rats induced by dimethylnitrosamine.%目的 探讨乙酰半胱氨酸镁对大鼠肝硬化门静脉高压血管活性物质和肝纤维化指标的影响.方法 以12 μg· kg-1二甲基亚硝胺制备大鼠肝硬化模型,肝硬化造模完成后分组实验,乙酰半胱氨酸镁盐按25、50、100 mg·kg-1剂量每日分别腹腔注射进行肝硬化治疗,给药8周后处死大鼠取材进行各项指标测定.通过病理切片的检查、肝组织转化生长因子β1、一氧化氮、总一氧化氮合酶和诱导型一氧化氮合酶的检测来评价乙酰半胱氨酸镁盐治疗肝硬化门脉高压的效果.结果 二甲基亚硝胺造模结束后,模型组大鼠HE与网格染色结果显示其肝硬化处于Ⅲ-Ⅳ期;乙酰半胱氨酸镁低、中和高剂量组肝硬化程度明显轻于模型组,淋巴细胞浸润和假小叶形成减轻;治疗低、中和高剂量组转化生长因子β1指标明显低于模型组(P＜0.05);与模型组比较,各治疗组的一氧化氮、总一氧化氮合酶和诱导型一氧化氮合酶明显降低(P＜0.05).结论 乙酰半胱氨酸镁具有独特的抗氧化性,能清除体内各种自由基,调节配体依赖信号传导和细胞生长,对二甲基亚硝胺引起大鼠肝硬化门静脉高压症具有明显地保护作用.