目的 从线粒体损伤的角度探讨ACLF存在的能量代谢障碍.方法 雄性SD大鼠随机分三组,ACLF组、NAC组各15只,正常组10只,制作ACLF模型,NAC灌胃作抗氧化模型.留肝组织及血液标本,梯度离心分离线粒体,ELLSA法测线粒体Cyt C、荧光酶标法测MPTP RFU;酶标法测肝组织、血浆GSH、MDA、SOD.结果 ACLF组肝组织、血浆MDA明显高于正常及NAC组(P＜0.05);NAC组MDA高于正常组(P ＜0.05);ACLF组血浆GSH、SOD低于正常及NAC组(P＜0.05);三组Cyt C为正常组最高、ACLF组最低(P＜0.05);三组MPTP为ACLF组最高、正常组最低;MPTP与Cyt C、ATP呈负相关(r=-0.858,-0.799);Cyt C与ATP呈正相关(r=0.78).结论 ACLF大鼠处于氧化应激损伤,Cyt C外流入胞质可能是ACLF发生能量代谢障碍的原因之一;NAC可在一定程度上提高肝细胞的抗氧化能力.%Objective To investigate energy metabolism with mitochondrial damage through building acute on chronic liver failure (ACLF) rat model.Methods Male SD rats were randomly divided into three groups,both ACLF and NAC group had 15 rats in each,control group had 10 rats.Rat model was treated with antioxidant therapy.Cytochrome C (Cyt C) was determined by using ELLSA and Cyt C,and fluorescence microplate method was used to determine mitochondrial permeability transition pore (MPTP) relative fluorescence unit (RFU) ; ELISA method was used to determine liver tissue and plasma glutathione (GSH),malonaldehyde (MDA),and superoxide dismutase (SOD).Results Levels of MDA in both liver and plasma are higher than that in normal and NAC groups (P ＜0.05) ; Levels of MDA in NAC group was higher than in normal group (P ＜ 0.05) ; levels of GSH and SOD of plasma in ACLF group is lower than in normal and NAC group (P ＜ 0.05) ; Cyt C in the normal group was the highest and ACLF group the lowest (P ＜0.05) ; ACLF group had the highest MPTP and normal group the lowest; MPTP with Cyt C and ATP content was negatively correlated (r =-0.858,-0.799) ; Cyt C and ATP content was positively correlated (r =0.78).Conclusion There is oxidative stress in ACLF rats; the flowing of Cyt C may be one of reasons of abnormal energy metabolism in ACLF; exogenous supply of NAC can improve the antioxidant capacity of the liver cells.
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