大鼠低氧低温睡眠剥夺后血清TNF-α和IL-6水平变化的研究

摘要

Objective To investigate the concentration of TNF-a and IL-6 in blood serum level in sleep deprivation model of SD rats under hypoxia and hypothermia conditions. Methods Healthy adult SD rats were randomly allocated into five groups including antitheses group ( CC group ), sleep deprivation group( SD0 ) and sleep deprivation group at the 1st day, the 4th day and the 7th day ( SD, ,SD4 ,SD7 ) under hypoxia and hypothermia conditions. ELISA were used to test the concentration of the blood serum of TNF-a and IL-6. Results ( 1 )Compared with the CC group, the concentration of blood serum TNF-a in SD, ,SD4 and SD7 group rise significantly( P <0. 05 andP <0. 01 );there was no significant different in TNF-a concentration between SD0 and SD, group( P >0.05 ), while the TNF-a concentration in SD4 and SD7 group rise significantly( P < 0.01 ). The serum TNF-a concentration tends to rise as the sleep deprivation prolongs.( 2 )Compared with the CC group, the concentration of blood serum IL-6 in SD, ,SD4 and SD7 group rise significantly( P <0.01 );compared with the SD0 group, the concentration of blood serum IL-6 in SD, , SD4 and SD7 group rise significant^ P <0. 01 ). The concentration of blood serum IL-6 tends to rise first and then decline as the sleep deprivation prolongs. Conclusion ( 1 )The expression of TNF-a and IL-6 in blood serum of the sleep deprivation SD rats were related with degree and duration of sleep deprivation. ( 2 )The rise in TNF-a and IL-6 caused by hypoxia, hypothermia and sleep deprivation might protect the brain cells in the short run,but long-term continued increase of its level would like to injure the neuron.%目的 研究低氧低温睡眠剥夺条件下大鼠血清肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)浓度变化的意义.方法 选用成年健康SD大鼠,随机分为5组,每组5只:正常对照组(CC组)、单纯睡眠剥夺组(SD0组)、低氧低温环境下睡眠剥夺1d组(SD1组)、睡眠剥夺4d组(SD4组)、睡眠剥夺7d组(SD7组).分别检测血清TNF-α和IL-6的浓度.结果 (1)TNF-α水平:与CC组比较,SD1组、SD4组、SD7组TNF-α水平均显著升高,差异有统计学意义(P<0.05,P<0.01);与SD0组比较,SD1组差异无统计学意义(P>0.05),而SD4组、SD7组水平显著升高,差异有统计学意义(P<0.01).随低氧低温睡眠剥夺时间的延长,TNF-α水平呈现逐步升高趋势.(2)IL-6水平:与CC组比较,SD1组、SD4、SD7组IL-6水平显著升高,差异有统计学意义(P<0.01);与SD0组比较,SD1组、SD4组、SD7组水平均显著升高,差异有统计学意义(P<0.01).随低氧低温睡眠剥夺时间的延长,IL-6水平呈现先升高后下降的趋势.结论 (1)大鼠血清TNF-α和IL-6的表达水平与低氧低温睡眠剥夺的严重程度及持续时间有关.(2)低氧低温睡眠剥夺引起的血清TNF-α和IL-6的增高,短期可能对机体脑细胞具有应激性保护作用,而长期持续增高可能对神经元有损伤作用.