Objective To observe the expression of different phase's relative inflammatory factors in serum in rats with limb ischemia-reperfusion, and discuss the clinical significance of its' variance tendency. Methods Seventy SD-rats were randomly divided into two groups: sham operated group and ischemia-reperfusion group ( IR group ), model of rats' right limb ischemia-reperfusion,blood of each group was collected respectively 1,3,6,9,12,15,18 hours after reperfusion, the contents of TNF-a, IL-1 β, IL-6 were measured by enzyme-linked immunosorbent assay( ELISA ). Results The contents of TNF-α, IL-1β, IL-6 of all phase's IR groups except the group of 1 hours after reperfusion were higher than those of sham operated group( P <0.05 ). The content of IR group's TNF-α was increased from postperfusion-3 hours, at peak time on postperfusion-9 hours and postperfusion-12 hours, and there was significant difference ( P < 0.05 ). The contents of IR group's IL-1 β, IL-6 were increased with time, and at peak time on postperfusion-12 hours( P < 0.05 ). No significant difference was found in the expression change of TNF-α, IL-1 β, IL-6 in all phase's sham operated groups ( P > 0. 05 ). Conclusion The inflammatory factors involve in the occurrence and development of early ischemia-reperfusion injury,and each inflammatory factor's expression in different phases of ischemia-reperfusion is of disparity.%目的 通过观察大鼠肢体缺血再灌注后不同时点血清中相关炎性因子的表达特点,探讨其变化的临床意义.方法 健康SD大鼠70只按数字表法随机分为假手术组及缺血再灌注组,采用大鼠右下肢缺血再灌注模型,各组分别在再灌注后1 h、3 h、6 h、9 h、12 h、15 h、18 h取血,ELISA法测定TNF-α、IL-1β、IL-6的含量.结果 除了再灌注1 h组外,IR组各时点血清TNF-α、IL-1β、IL-6水平均高于假手术组(P＜0.05).其中,IR组TNF-α含量自再灌注3 h后递增,至9 h和12 h达高峰,差异有统计学意义(P＜0.05);IR组中IL-1β、IL-6表达随时间而升高,于12 h达高峰(P＜0.05)后水平呈下降趋势.假手术组中各时点血清TNF-α、IL-1β、IL-6水平变化差异无统计学意义(P＞0.05).结论 炎性因子参与了早期缺血再灌注损伤的发生发展过程,不同炎性因子在缺血再灌注过程中表达的时相存在差异.
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