白介素-1β基因-511和＋3954多态性与男性煤矿职工慢性阻塞性肺疾病的相关性

摘要

Objective To investigate the relationship between interleukin-1 beta ( IL-1β)-511 gene and +3954 pol-ymorphism and male coal mine workers with chronic obstructive pulmonary disease ( COPD) .Methods From January 2009 to June 2013 , collected the Hebei United University affiliated Kailuan General Hospital Outpatient Department ’ s respiration screening COPD patients (234 cases of coal mine workers as coal mine workers COPD group ) and 250 cases of healthy con-trols (healthy control group), using enzyme linked immunosorbent assay (ELISA) to detect serum IL-1βlevel, polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method to detect -511C/T, genotype +3954C/T sites. Results The coal mine worker group COPD serum IL-1βlevel was (3.98 ±0.42 ) ng/ml, the healthy control group was (2.76 ±0.18) ng/ml, with statistically significant difference between the 2 groups ( P <0.01).Coal mine worker group COPD IL-1β-511 CC, CT and TT genotypes were 20.1%, 62.8%and 17.1%, healthy control group were 21.6%, 60.4%and 18.0%, compared between the 2 groups, there was no statistical significance ( P >0.05).Coal mine worker group COPD IL-1β+3954 , CC, CT and TT genotypes were 83.8%, 16.2%and 0, healthy control group were 86.8%, 13.2% and 0, compared between the 2 groups, there was no statistical significance ( P >0.05),there were no significant differences in the frequency of 2 groups’ IL-1β-511, +3954 allele ( P >0.05).Conclusion Coal mine worker group COPD serum IL-1βlevels higher than the healthy control group .IL-1βand genotype frequency of -511 , the incidence of +3954 and allele fre-quency have no obvious correlation with coal mine workers ’ COPD.%目的：探讨白细胞介素-1β（IL-1β）基因-511和＋3954多态性与男性煤矿职工慢性阻塞性肺疾病（ COPD）的相关性。方法收集2009年1月-2013年6月河北联合大学附属开滦总医院呼吸科门诊筛查的234例煤矿职工COPD患者（煤矿职工COPD组）及250例同期健康对照人群（健康对照组），用酶联免疫吸附试验（ELISA）检测血清IL-1β水平，应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测-511C/T，＋3954C/T位点的基因型。结果煤矿职工COPD组的血清IL-1β水平为（32．98±0．42）ng/ml，健康对照组为（2．76±0．18）ng/ml，2组间差异具有统计学意义（ P ＜0．01）。煤矿职工COPD组IL-1β-511的CC、CT和TT基因型频率分别为20．1％、62．8％和17．1％，健康对照组分别为21．6％、60．4％和18．0％，2组相比差异无统计学意义（ P ＞0．05）。煤矿职工COPD组IL-1β＋3954 CC、CT和TT基因型频率分别为83．8％、16．2％和0，健康对照组分别为86．8％、13．2％和0，2组相比无统计学意义（ P ＞0．05）。2组人群IL-1β-511，＋3954等位基因频率比较差异无统计学差异（ P均＞0．05）。结论煤矿职工COPD组血清IL-1β水平高于健康对照组， IL-1β-511、＋3954的基因型频率与等位基因频率与煤矿职工COPD的发生无明显相关性。