左旋多巴对帕金森病模型大鼠结肠酪氨酸羟化酶和ɑ-突触核蛋白表达的影响

摘要

Objective To investigate the effect of levodopa (L-dopa) on Parkinson's disease (PD) rats colon tyrosine hydroxylase(TH)andtheexpressionofalpha-synuclein(ɑ-Syn).Methods 72healthySDratswererandomlydividedinto control group, model of PD group (model group),L-dopa treatment group (treatment group), each group was subdivided into the successful preparation of the model after 4 d, 8 d and 2 sub-groups, 12 rats in each group.Preparation of PD model rats were performed with subcutaneous injection of rotenone , immunohistochemistry and Western blotting blot were used to detect thecolonicTHandɑ-Synexpressionlevel.Results 1hfecaloutputandwatercontent:comparedwithcontrolgroup,therats in model group and the treatment group decreased significantly ( P <0.05); compared with the model group, rats in the treatment group were increased ( P <0.05); and compared with the treatment 4 d subgroups, treatment 8 d group was in-creased ( P <0.05).(1)Compared with the control group, model group and treatment group at each time point , colon TH positive cells and protein expression were significantly reduced , the expression of ɑ-Syn positive cells and protein were signifi-cantly increased ( P <0.05);(2)Compared with the model group, the treatment group at each time point, colon TH positive cells and protein expression were increased , the expression of ɑ-Syn positive cells and protein was significantly reduced ( P <0.05);(3)Compared with the 4 d treatment group, TH positive cells and protein expression was elevated in 8 d treatment subgroups ( P <0.05),ɑ-Syn positive cells and protein were decreased ( P <0.05).Conclusion The mechanism of PD model rat colonic dysfunction may related with reduced dopamine and increased ɑ-Syn level,L-dopa may by increasing dopa-mine and reduce the level of ɑ-Syn thus improve colonic dysfunction in PD rats .%目的：探讨左旋多巴（L-dopa）对帕金森病（PD）模型大鼠结肠酪氨酸羟化酶（TH）及ɑ-突触核蛋白（ɑ-Syn）表达的影响。方法健康SD大鼠72只随机分为对照组、帕金森病模型组（模型组）、左旋多巴治疗组（治疗组），各组随机再分为模型制备成功后4 d、8 d 2个亚组，各12只。采用大鼠颈背部皮下注射鱼藤酮制备帕金森病模型，免疫组化法和Westernblotting蛋白印迹法检测结肠TH和ɑ-Syn表达水平。结果1h粪便排出量及含水量：与对照组比较，模型组、治疗组大鼠显著降低（ P ＜0噜．05）；与模型组比较，治疗组大鼠增加（ P ＜0．05）；与治疗4 d亚组比较，治疗8 d亚组增加（ P ＜0．05）。免疫组化法与Western blotting检测TH、ɑ-Syn比较：（1）与对照组比较，模型组、治疗组各时间点结肠TH阳性细胞及蛋白表达均明显减少，ɑ-Syn阳性细胞及蛋白表达均明显增加（ P均＜0．05）；（2）与模型组比较，治疗组各时间点结肠TH阳性细胞及蛋白表达均增多，ɑ-Syn阳性细胞及蛋白表达均明显减少（ P均＜0．05）；（3）与治疗4 d亚组比较，治疗组治疗8 d亚组的TH阳性细胞及蛋白表达升高（ P ＜0．05），ɑ-Syn阳性细胞及蛋白降低（ P ＜0．05）。结论 PD模型大鼠结肠功能障碍的机制可能与多巴胺的减少及ɑ-Syn水平的增多有关，左旋多巴可能通过增加多巴胺和减少ɑ-Syn水平改善PD大鼠结肠功能障碍。