目的：观测非小细胞肺癌(NSCLC)患者血清和呼出气冷凝液中白介素-17(IL-17)、血管内皮生长因子( VEGF)水平及其在肺癌发病机制中的作用。方法收集NSCLC患者39例为NSCLC组,同期健康体检者20例为健康对照组,采集空腹血清和呼出气冷凝液( EBC)标本,采用ELISA方法检测血清及EBC中IL-17、VEGF水平,并进行相关性分析。结果(1) NSCLC组血清IL-17及VEGF水平分别为(27.34±8.12)pg/ml及(42.72±7.69)pg/ml,均明显高于健康对照组的IL-17(16.23±4.22)pg/ml、VEGF(24.64±5.17)pg/ml( P均<0.01)。(2)NSCLC组EBC中IL-17水平和VEGF水平分别为(5.96±1.51)pg/ml,(22.73±4.26)pg/ml,均高于健康对照组的(1.96±0.49)pg/ml、(13.31±4.61)pg/ml( P均<0.01)。(3)NSCLC患者外周血清和EBC中IL-17、VEGF水平随临床分期进展呈上升趋势( P <0.01)。(4)NSCLC患者血清中IL-17水平与VEGF水平呈正相关性( r =0.594, P <0.01),EBC中IL-17水平与VEGF亦呈正相关( r =0.704, P =0.000)。血清中IL-17、VEGF水平与临床分期呈正相关( r =0.811、0.722, P均<0.01)。 EBC中IL-17、VEGF水平与临床分期亦呈正相关( r =0.843、0.789, P均<0.01)。结论 IL-17参与NSCLC的发病过程,可能和其促进VEGF的表达,进一步促进肿瘤生长有关。 EBC中IL-17水平的检测可作为NSCLC患者监测病情变化的无创性指标。%Objective To observe interleukin 17 (IL-17) and vascular endothelial growth factor (VEGF) in serum and exhaled breath condensate( EBC) in non-small cell lung cancer ( NSCLC) ,and it role in the pathogenesis of lung cancer. Methods Collected 39 NSCLC patients as NSCLC group, 20 cases of healthy subjects as healthy control group, collected fasting serum and EBC specimens, using ELISA method for the detection of serum and EBC IL-17 and VEGF levels, and cor-relation analysis were performed. Results (1) In the NSCLC group, the serum levels of IL-17 and VEGF levels were (27. 34 ± 8. 12) pg/ml and (42. 72 ± 7. 69) pg/ml,which were significantly higher than those in healthy control group of the IL-7:(16. 23 ± 4. 22) pg/ml, VEGF:(24. 64 ± 5. 17) pg/ml ( P <0. 01). (2) The NSCLC group, in EBC, IL-17 concentration and VEGF concentration were (5. 96 ± 1. 51) pg/ml and (22. 73 ± 4. 26) pg/ml respectively, which were higher than in the healthy control group’s (1. 96 ± 0. 49) pg/ml, (13. 31 ± 4. 61) pg/ml ( P <0. 01). (3) NSCLC’s EBC in the peripheral blood, IL-17 and VEGF levels were increased along with clinical stage progress increased ( P <0. 01). (4) In NSCLC, ser-um IL-17 levels and VEGF levels were positively correlated ( r =0. 594, P < 0. 01), EBC IL-17 levels and VEGF was also positively correlated ( r =0. 704, P =0. 000). The serum level of VEGF and IL-17 was positively correlated with clinical stage( r =0. 811, r =0. 722, P <0. 01). IL-17, VEGF level and clinical stage was positively correlated to EBC ( r =0. 843, r =0. 789, P <0. 01). Conclusion IL-17 participates in the pathogenesis of NSCLC. It may promote VEGF expres-sion to further promote tumor growth. EBC IL-17 level can be used as a noninvasive index for monitoring the disease condition of NSCLC.
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