不同剂量盐酸吡格列酮对糖尿病大鼠尿podocalyxin排泄的影响

摘要

Objective To observe the effect of different dosages of hydrochloride pioglitazone (PIO) on the excretion of urinary podocalyxin (PCX) in STZ-induced diabetic rats. Methods Diabetic rats were randomized into the following four groups: diabetic rats without treatment (Model group, n = 8), and PIO 10, 20, and 30 mg/(kg·d) groups (DR1, DR2, and DR3 groups, n = 8 in each) treated by intragastric administration. UAlb, Cr, and urinary PCX were measured on the first day and at the end of the 4th and 8th weeks respectively. Results (1) At the end of the 4th week, compared with the model group, the level of urinary albumin-to-creatinine ratio (UACR) was significantly decreased in both DR2 and DR3 groups (P<0. 01). However, there was no statistical difference in the level of urinary podocalyxin-to-creatinine ratio (UPCR) among the four groups. (2) At the end of the 8th week, compared with the model group, the level of UPCR was significantly reduced in both groups DR2 and DR3 (P< 0. 01), there was no statistical difference between the DR1 and model groups. The level of UACR was significantly lower in the PIO groups than in the model group (P<0. 01), while the level of UACR was significantly lower in the DR2 and DR3 groups than in the DR1 group (P<0. 05). (3) UPCR was positively correlated with UACR (r=0. 86, P<0. 01). Conclusion PIO can restrain the loss of PCX in the urine with a dose and time dependent manner, which may play a protective role for the kidney in STZ-induced diabetic rats.%目的 观察不同剂量盐酸吡格列酮(PIO)对糖尿病大鼠尿podocalyxin(PCX)排泄的动态影响. 方法 糖尿病大鼠分为DM组及PIO组(DR1、DR2、DR3组,分别给予PIO 10、20、30 mg/(kg·d).分别于0、4、8周末监测UAIb、Cr、尿PCX. 结果 (1)4周末,DR2和DR3组尿白蛋白肌酐比(UACR)较DM组显著降低(P＜0.01),PIO各组尿PCX肌酐比(JpCR)与DM组相比差异无统计学意义.(2)8周末,DR2、DR3组UPCR较DM组显著降低(P＜0.01),DR1组与DM组UPCR差异无统计学意义；PIO各组UACR均较DM组明显降低(P＜0.01),DR2组和DR3组UACR较DR1组明显降低(P＜0.05).(3)UPCR与UACR呈正相关(r=0.86,P＜0.01). 结论 吡格列酮可抑制糖尿病大鼠PCX随尿排泄,并具有一定的剂量和时间依赖性.