尖锐湿疣组织中人乳头瘤病毒基因型的检测

摘要

Objective To study the infection and distribution of human papillomavirus (HPV) genotypes in condyloma acuminata (CA) specimens.Methods 300 samples from CA patients were examined for the identification of HPV genotypes with flow-through hybrization and gene chip.Results In the 300 patients studied,265 cases were showing HPV positive,the total positive rate was 88.34％,the positive rate of single type of HPV was 68.68％,the positive rate of multiple types of HPV was 31.32％.21 genotypes were found by HybriMax in total 21 HPV genotypes in 300 clinic samples.The most common subtypes of HPV was HPV6,followed by HPV11,HPV16,HPV18,HPV52,HPV58 and etc.Conclusion HPV6,HPV11 of low-risk HPV,and HPV16,HPV18 of high-risk HPV are the most popular genotypes in CA,the majority of high-risk HPV genoytpes exist with multiple-genotypes.%目的 探讨尖锐湿疣组织中人乳头瘤病毒(HPV)基因型的感染情况.方法 采用核酸分子快速导流杂交基因芯片技术,对300例尖锐湿疣患者皮损组织进行HPV感染分型的检测.结果 300份尖锐湿疣组织标本中HPV阳性265例,检出率是88.34％;其中单一感染阳性率达到68.68％(182/265),多重感染达到31.32％(83/265).265份尖锐湿疣组织标本中检测出21种HPV亚型,各型别检出率由高到低前6位依次是HPV6(128例,48.31％)、HPV11(90例,33.97％)、HPV16(33例,12.46％)、HPV18(22例,8.31％)、HPV52(22例,8.31％)和HPV58(22例,8.31％).结论 尖锐湿疣以低危型HPV6,HPV11感染和高危型HPV16,HPV18感染为主,高危型HPV感染多以混合感染的形式存在.