脓毒症大鼠血脑屏障通透性及脑组织 ZO -1表达的实验研究

摘要

Obj ective To observe the changes on blood -brain barrier permeability and brain expression of ZO -1 in sepsis rats.Methods Fifty SD rats were divided into sham operation group (n=10) and model group (n =40).The model rats were further divided into 6, 12, 24, 48 h subgroups (n=10).The sepsis rat model was developed by CLP .The blood-brain barrier permeability was detected by spectrophotometric analysis of EB .The expression of ZO -1 mRNA and protein was detected by RT-PCR and Western -blot, respectively.Results The EB contents of each time point groups were higher than sham operation group (P<0.01).The expression of ZO-1 in each time point groups were lower than sham operation group (P <0.01).And the level of ZO -1 expression was continuously decreased during the development of sepsis .Conclusion The expression levels of ZO -1 mRNA and protein in sepsis rats are decreased significantly , and this may be one of the mechanisms of blood-brain barrier damage of sepsis .%目的：观察脓毒症大鼠血脑屏障通透性及脑组织胞质附着蛋白（ ZO-1）的变化。方法50只健康SD大鼠随机分为假手术组（ n＝10）及脓毒症模型组（ n＝40），脓毒症模型组又分为脓毒症制模后6、12、24、48 h 4个亚组，每组10只，采用盲肠结扎穿孔（ CLP）法制作脓毒症模型。于相应时间点处死取材，伊文思蓝（ EB）外渗分光光度分析法检测血脑屏障通透性；RT-PCR法测定脑组织ZO-1 mRNA表达、Western-blot免疫印迹方法检测ZO-1表达。结果脓毒症组各时间点大鼠脑内EB含量均明显高于假手术组（ P＜0．01）；脓毒症各时间点组大鼠脑内ZO-1 mRNA、ZO-1的表达均明显下降，与假手术组比较差异有统计学意义（P＜0．01），且随着脓毒症时间延长下降越明显。结论脓毒症大鼠脑内ZO-1 mRNA、ZO-1的表达均显著下降，可能是脓毒症血脑屏障受损的机制之一。