AIM: To observe the effects of interleukin - 1(3 (IL-lβ) on mRNA expression of osteoprote-rngerin ( OPG) and receptor activator of NF-kappa B ligand ( RANKL) in human periodontal ligament fibroblasts ( HP-DLF) . METHODS: Primary HPDLFs were cultured according to the method of tissue explant in vitro and identified by immunohistochemistry. HPDLFs from passages 4 through 8 were used for experiments. HPDLFs were exposed to IL-1 (3 of different concentrations (0, 0. 01, 0. 1, 1, 10, 100 ng/mL) , respectively. The mRNA expression of OPG and RANKL in HPDLF was detected using semi quantitative reverse transcriptase polymerase chain reaction ( RT- PCR) a-nalysis. RESULTS: IL-1β up-regulated OPG mRNA levels in HPDLF in a dose-independent manner. 0.01 ng/mL IL -lβ increased the mRNA expression of OPG and the effect reached maximum at 0. lng/mL. OPG mRNA levels de-creased gradually with the increase of IL-1β concentration above lng/mL. IL-lβ up-regulated the mRNA levels of RANKL and RANKL/OPG ratie in HPDLF in a dose-dependent manner. Except for 10 and 100 ng/mL IL-lp. the rest concentrations of IL-lβ increased the mRNA expression of RANKL and the RANKL/OPG ratio markedly. CON-CLUSION : IL- 1 β can up-regulate mRNA expression of RANKL and increase RANKL/OPG ratio in human periodon-tal ligament cells, therefore it can regulate osteoclast differentiation, stimulates bone resorption and lead to the alveolar bone destruction.%目的:以体外培养的人牙周膜成纤维细胞(HPDLF)为研究对象,观察不同浓度的白细胞介素-1β(IL-1β)对人牙周膜成纤维细胞(HPDLF)中骨保护素(OPG)、核因子κB受体活化剂配体(RANKL) mRNA表达的影响.方法:组织块法体外原代培养HPDLF并鉴定,以第5代HPDLF作为实验靶细胞,分别用不同浓度(0、0.01、0.1、1、10、100 ng/mL)的IL-1β进行干预,半定量逆转录聚合酶链反应(RT-PCR)检测HPDLF中OPG、RANKL mRNA的表达.结果:IL-1β在0.01 ～ 10 ng/mL浓度范围内能明显上调HPDLF中OPG mRNA的表达(P＜0.05),并在0.1 ng/mL时上调作用达到最大(P＜0.05).此后,随着IL-1β浓度的增加,OPG mRNA的表达量逐渐减少.IL-1β在0.01 ～ 100 ng/mL浓度范围内均可明显上调HPDLF中RANKL、RANKL/OPG mRNA的表达(P＜0.05),且呈剂量依赖性,即随着IL-1β浓度增加,HPDLF中RANKL、RANKL/OPG mRNA的表达也逐渐增加,各浓度组两两比较除10 ng/mL与100 ng/mL相比无显著性差异外,其余各组间差异均有统计学意义(P＜0.05).结论:IL-1β可促进HPDLF中RANKL mRNA的表达,上调RANKL/OPG mRNA的比值.
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