基于均匀设计的复方金银花中药漱口水有效组分的配伍研究

摘要

AIM: To sift the optimal ratio of artemisia capillaris thunb(ACT, X1), chrysanthemum(CHR, X2),honeysuckle(HDN,X3),angelica dahurica and asarum sieboldii(AD and AS, X4)by uniform design. METH-ODS:According to the uniform design and the U8(85) uniform design table,ACT,CHR,HON,AD and AS were used as the factors and 8 concentrations of each medicine as the levels. By evaluating the antibacterial activity against Porphy-romonas gingivalis(P.gingivalis),the inhibition rate of biofilm formation and the toxicity on human gingival fibroblasts were explored,the optimal ratio of the 5 drugs was sieved. RESULTS: The above-mentioned compounds all showed inhibitory effects on the growth and biofilm formation of P.gingivalis. Binomial regression was applied to obtain the opti-mal ratio of the 5 drugs and the equation was Y = -0.9918 -0.0044X2-0.0002X1X2-0.0005X1X3+ 0.0004X1X4+0.0017X2X3. In addition,the optimal ratio was 55 mg/mL ACT,45 mg/mL for,40 mg/mL HON,40 mg/mL for AD and AS(33:17). CONCLUSION:In the compound of honeysuckle traditional chinese medicine mouthwash the optimal ratio is 55 mg/mL for ACT,45 mg/mL for CHR,40 mg/mL for HON,40 mg/mL for AD and AS(33:17).%目的:筛选茵陈(X1)、野菊花(X2)、金银花(X3)、白芷及细辛(X4)在复方金银花中药漱口水中的最佳配伍.方法:利用均匀设计的方法,并根据U8(85)均匀设计表,以茵陈、野菊花、金银花和细辛+白芷为因素,以各药物8个浓度为水平,以对牙龈卟啉单胞菌(Pg)的抑菌活性、生物膜形成抑制率及牙龈成纤维细胞毒性作用为指标,筛选5种药物的最佳配伍.结果:各复方对Pg的生长及生物膜形成均具有抑制作用.将数据进行二项式逐步回归,得出回归方程为 Y = - 0. 9918 - 0. 0044X2-0.0002 X1X2-0.0005X1X3+0.0004X1X4+0. 0017X2X3;对回归方程进行优化后,获得各个因素的最佳配伍为:茵陈55 mg/mL,菊花45 mg/mL,金银花40 mg/mL,白芷+细辛(33:17)40 mg/mL.结论:以茵陈、野菊花、金银花、白芷及细辛为有效成分的复方能有效抑制 Pg,其最佳配伍为茵陈55 mg/mL,菊花45 mg/mL,金银花40 mg/mL,白芷+细辛(33:17)40 mg/mL.