瑞舒伐他汀对胶质瘤U87细胞迁移、侵袭的抑制作用

摘要

Objective To investigate the inhibitory effect of rosuvastatin on the migration and invasion of U87 glioma cells and its related mechanisms. Methods Cultured U87 cells were treated with 0, 5, 10 and 20 μmol/L rosuvastatin for 24,48 and 72 h. Cell viability was measured by CCK-8 assay. Migration ability of the cells was detected by scratch assay,and invasion ability of the cells was detected by Transwell assay. The expression of matrix metalloproteinase 2(MMP2),MMP9 and the Wnt/β-catenin signaling pathway-related proteins was detected by Western blot assay. Results Compared with the control group,the viability of U87 cells in the 5,10 and 20 μmol/L rosuvastatin groups was decreased (P < 0.01)in a time- and dose-dependent manner. Migration and invasion abilities of the cells were decreased(P <0.01). In addition,the expression levels of MMP2,MMP9,Wnt1,Wnt3a,Wnt7a,and β-catenin were decreased as well (P < 0.01). Conclusions Rosuvastatin can significantly inhibit the migration and invasion abilities of U87 glioma cells, probably related to the blocking of Wnt/β-catenin signaling pathway.%目的 探讨瑞舒伐他汀对胶质瘤细胞U87迁移、侵袭的抑制作用及相关机制.方法 0、5、10、20 μmol/L的瑞舒伐他汀作用U87细胞24、48、72 h,CCK-8法检测细胞活力,划痕实验检测细胞迁移能力,Transwell实验检测细胞侵袭能力,Western blot检测基质金属蛋白酶MMP2、MMP9及Wnt/β-catenin信号通路相关蛋白表达.结果 与对照组比较,5 μmol/L组、10 μmol/L组、20 μmol/L组U87细胞活力降低(P < 0.01),呈时间及剂量依赖性,细胞迁移及侵袭能力降低(P< 0.01),MMP2、MMP9、Wnt1、Wnt3a、Wnt7a、β-catenin表达量下调(P< 0.01).结论 瑞舒伐他汀能显著抑制胶质瘤细胞U87迁移及侵袭能力,可能与阻断Wnt/β-catenin信号通路有关.