CtBP2在食管鳞状细胞癌中的表达及其临床意义

摘要

Objective:To explore the expression of C-terminal binding protein 2 (CtBP2) in human esophageal carcinoma and its relationship with clinicopathological parameters and survival. Methods:The expression levels of CtBP2 in eight cases of fresh frozen specimens of esophageal squamous cell carcinoma (ESCC) and the adjacent esophageal tissues were detected by Western blot. Immuno-histochemistry was used to detect CtBP2 expression in 90 samples of ESCC tissues and adjacent non-tumor tissues. Based on patient in-formation and follow-up data, the correlation of CtBP2 expression with patients' clinicopathological characteristics and overall survival was further evaluated using Fisher's exact test and Kaplan-Meier method, respectively. Results: Immunohistochemistry and Western blot analysis showed that CtBP2 expression in tumor tissues was higher than that in adjacent non-tumor tissues. CtBP2 expression was significantly correlated with histologic grade (P=0.002) and depth (P=0.032). Kaplan-Meier analysis demonstrated that high CtBP2 ex-pression was correlated with a short survival time. Conclusion:CtBP2 expression was upregulated in ESCC tissues, indicating that it may play a role in the oncogenesis and development of ESCC.%目的:研究羧基末端结合蛋白2(C-terminal binding protein 2,CtBP2)在食管鳞状细胞癌组织中的表达情况及其与食管癌发生发展的关系.方法:Western blot法检测8对食管鳞状细胞癌新鲜冰冻组织、免疫组化法检测90例食管鳞状细胞癌石蜡切片组织中CtBP2表达情况,结合临床病理和随访资料分析CtBP2表达与患者临床病理参数及总生存率的关系.结果:两法检测结果均显示CtBP2食管鳞状细胞癌组织中的表达明显高于对应的癌旁组织,且CtBP2的表达水平与食管癌的组织学分级(P=0.002)、浸润深度(P=0.032)相关,而与年龄、性别等参数无相关性.Kaplan-Meier分析结果显示,CtBP2高表达组患者的总体生存率明显低于CtBP2低表达组患者.结论:CtBP2在食管鳞状细胞癌组织中表达显著上调,提示其可能与食管鳞状细胞癌的发生发展相关.