姜黄素对肾小球系膜细胞过氧化物酶体增殖物激活受体γ基因表达的影响

摘要

目的:观察姜黄素对肾小球系膜细胞过氧化物酶体增殖物激活受体γ(PPAR-γ)mRNA基因表达水平的影响.方法:将高浓度葡萄糖液及不同浓度的姜黄素液与肾小球系膜细胞共孵育,用逆转录聚合酶链反应(RT-PCR)法,检测各组细胞 PPAR-γmRNA表达强度.结果:单纯高浓度葡萄糖组肾系膜细胞PPAR-γmRNA表达量(0.394±0.026)显著低于正常对照组(0.995±0.016),P<0.01;当姜黄素的浓度达100 mg/L时,其PPAR-γmRNA积分吸光度比值为0.701±0.04,高于高浓度葡萄糖+姜黄素(25 mg/L)组(0.541±0.018),显著高于高浓度葡萄糖+姜黄素(6.25 mg/L)组(0.432±0.024),P<0.01.结论:姜黄素拮抗高浓度葡萄糖对肾系膜细胞PPAR-γ基因表达的抑制作用,上调PPAR-γmRNA的表达水平.%Objective:Observe effects of Curcumin on gene expression of the peroxisome proliferator-activate receptors gamma (PPAR-γ) mRNA in glomerulus mesangial cells. Methods: High glucose concentration and Curcumin solutions of different concentrations were incubated with glomerulus mesangial cells by using reverse transcription-polymerase chain reaction (RT-PCR)to detect expression of PPAR-γmRNA of cells. Resuls: The expression of PPAR-γmRNA in glomerulus mesangial cells of the group of pure high glucose concentration was lower than the normal group (0. 394 ± 0. 026 vs. 0. 995 ± 0. 016, P＜0. 01 ).When concentration of Curcumin reaches 100mg/L, the ratio of integral optical density of its PPAR-γmRNA was, higher than that of Gurcunin 25 mg/L group (0. 701 ± 0.04 vs. 0. 541 ± 0. 018, P＜0.01 ) and it was significant higher than that of Curcumin 6. 25 mg/L group (0. 432 ± 0. 024, P＜0.01 ). Conclusions: Curcumin will antagonize inhibition effect of high glucose concentration on gene expression of PPAR-γ in glomerulus mesangial cells, and increase the expression level of PPAR-γmRNA.