调节性T细胞在乙型肝炎慢性化中的免疫调控作用

摘要

目的 探讨乙型肝炎慢性化的免疫调控机制.方法 用酶联免疫吸附实验测定慢性乙型肝炎患者(CHB)的辅助性T淋巴细胞(Th1/Th2)细胞因子IFN-γ、IL-2、IL-4和IL-10的血清浓度;同时用流式细胞术检测CHB的调节性.T细胞(Treg)在外周血中的分布频率.结果 与健康对照组相比,CHB组血清IL-2明显减低(P<0.05),而IL-4和IL-10均明显升高(P<0.05);CHB外周血Treg频率显著高于健康对照组(P<0.05).结论 CHB组调节性T细胞增高,这可能是HBV感染易于慢性化的免疫机制之一.%Objective To investigate the immunopathogenesis of chronic hepatitis B vires (HBV) infection.Methods Serum T helper lymphocyte ( Thl ) cytokines ( IFN-γ, IL-2) and Th2 cytokines ( IL-4, IL-10 ) were measured in patients with chronic HBV infection, and the frequency of CD4 + CD25 + Foxp3 + were investigated by flow cytometry. Results Serum IL-2 level was significantly decreased, but IL-4 or IL-10 level was significantly increased and the production of Th2 cytokines was more predominant in CHB patients as compared to healthy controls( P ＜ 0.05 ).Meanwhile, CHB patients presented a higher circulating T regulatory cell frequency thanhealthy controls did. Conclusion An enhanced Th2 response and higher circulating T regulatory cell frequency lies in HBV infection patients,which may be responsible for the infection chronicity.