FISH技术在鉴别上尿路尿道上皮癌与良性疾病引起血尿中的作用

摘要

目的 探讨荧光原位杂交技术(fluorescence in situ hybridization,FISH)作为非创性分子检查方法,用于鉴别上尿路尿道上皮癌(upper urinary tract urothelial carcinoma,UUT-UC)和良性泌尿系统疾病引起的血尿.方法 运用一组荧光标记的DNA探针检测34例UUT-UC患者和33例泌尿系统良性疾病患者血尿脱落细胞中3、7、9、17号染色体异常,并对结果进行分析.结果 34例UUT-UC患者血尿中25例(73.5%)检测出染色体异常,而良性疾病患者血尿脱落细胞检测出2例发生染色体异常(6.1%)(P<0.001).结论 FISH检测脱落细胞3、7、9、17染色体异常,可以作为一种非创性方法用于鉴别UUT-UC和良性疾病引起的血尿.%Purpose To evaluate the clinical utility of a fluorescence in situ hybridization ( FISH ) assay as a non-invasively molecular test to distinguish urothelial carcinoma in upper urinary tract ( UUT-UC ) from benign lesions presenting with hematuria. Methods The chromosomal abnormalities of chromosomes 3,7, 17, and 9 ( pi 6 ) in hematuria specimens from 34 patients with UUT-UC and 33 patients with benign disorders were detected by using a set of fluorescently labeled DNA probes. The abnormalities of chromosomes were determined and analyzed between UUT-UC and benign disorders. Results The abnormalities of chromosomes were detected in 25 out of 34 ( 73. 5% ) patients with UUT UC and in 2 out of 33 ( 6. 1 % ) patients with benign disorders ( P ＜ 0. 001 ). Conclusions FISH assay of chromosomes 3, 7, 9, and 17 performed on exfoliated cells from voided urine specimens could be used as a non-invasive method to distinguish urothelial carcinoma in upper urinary tract from benign disorders presenting with hematuria.