子宫内膜样腺癌中TBX2、PAX9的表达及其相关性

摘要

目的 探讨TBX2、PAX9在正常子宫内膜、子宫内膜增殖症和子宫内膜样腺癌(endometrioid adenocarcinoma,EA)组织中的表达及临床病理学意义.方法 采用组织芯片技术和免疫组化检测30例正常子宫内膜组织、30例单纯性增生内膜、30例复杂伴不典型性增生内膜、82例EA组织中TBX2、PAX9蛋白的表达.结果 TBX2蛋白在子宫内膜样腺癌中的阳性表达率明显高于正常子宫内膜和子宫内膜增殖症(P均＜0.01),TBX2过表达与组织学分级、临床分期、浸润程度均有相关性(P＜0.01,P＜0.05,P＜0.01),与淋巴结转移无相关性(P＞0.05);PAX9蛋白在子宫内膜样腺癌中的阳性表达率明显高于正常子宫内膜、子宫内膜单纯性增生(P均＜0.01),而和复杂伴不典型性增生之间没有统计学意义(P＞0.05),在复杂伴不典型增生中表达明显高于正常子宫内膜和单纯性增生内膜(P均＜0.01),PAX9阳性表达与组织学分级、临床分期、浸润程度均有相关性(P＜0.01,P＜0.01,P＜0.05),但与淋巴结转移无相关性(P＞0.05).TBX2与PAX9蛋白呈正相关(rs=0.427,P＜0.01).结论 TBX2和PAX9均在子宫内膜样腺癌中发生、发展中发挥重要作用,联合检测其表达可为子宫内膜样腺癌的早期诊断、预后判断提供有价值的指标.%Purpose To investigate the relationship between the expression of TBX2 and PAX9 proteins in the development of endome-trial carcinoma. Methods The expression levels of TBX2, PAX9 proteins in endometrial adenocarcinoma tissues ( n = 82 ), endometri-al hyperplasia tissues ( n = 60 ) and normal tissues ( n = 30 ) were examined by tissue microarray technique and immunohistochemistry ( SP method ). Results The positive expression rates of TBX2 protein in normal endometrium, endometrial simple hyperplasia, endometrial atypical hyperplasia and endometrial carcinomas were gradually increased ( P <0. 01 ). The positive expression of TBX2 was related to the histological grade ( P <0. 01 ), FIGO stage ( P <0. 05 ) and depth of invasion ( P <0. 01 ), but not related to the lymph node metastasis ( P >0. 05 ). The positive of PAX9 protein in endometrial carcinomas was higher than that in normal endometrium, endometrial simple hyperplasia ( P <0. 01 ), but it was not related to that in endometrial atypical hyperplasia ( P >0. 05 ). The positivity of PAX9 was related to the histological grade ( P < 0. 01 ), FIGO stage ( P < 0. 01 ), depth of invasion ( P < 0. 05 ), but not to the lymph node metastasis ( P >0. 05 ). The expression of TBX2 protein was positively related to that of PAX9 ( rs =0. 427 , P <0. 01 ). Conclusion This study reveals that the abnormal expression of TBX2 and PAX9 takes part in the development and progression of endometrial adenocarcinomas, and that TBX2 may associate with regulations of PAX9. The results of this study support the idea that PAX9 and TBX2 may be important biomarkers in the detection of biological behavior and prognosis in endometrial adenocarcinoma.