Tumstatin对兔耳增生性瘢痕的抑制作用

摘要

目的 探讨肿瘤抑素(Tumstatin)对兔耳瘢痕组织生长情况的影响.方法 建立兔耳增生性瘢痕模型,用Tumstatin对兔耳腹侧建立的增生性瘢痕模型通过在体局部注射进行干预,随机设立Tumstatin治疗组、Tumstatin预防治疗组、曲安奈德对照组、生理盐水对照组;停止药物注射21天后各组同时于每只兔的双侧对称部位取材瘢痕组织,每一取材瘢痕组织均分成同样大小的四等份,分别进行组织形态学观察、CD34免疫组化方法测定血管密度的变化、观察血管内皮细胞与成纤维细胞凋亡的变化情况.结果 Tumstatin预防治疗组和Tumstatin治疗组中,新生毛细血管密度、内皮细胞活性以及成纤维细胞数量都较曲安奈德和生理盐水对照组明显降低,差异有统计学意义(P<0.05).结论 Tumstatin能够较曲安奈德更明显地抑制增生性瘢痕的生长,为Tumstatin用于防治增生性瘢痕的可行性提供了初步的理论依据.%Purpose To investigate the effect of tumstatin on hypertrophic scar model in the rabbit ear with intervention through local injection. Methods An animal model of hypertrophic scar on the rabbit ears was established and was intervened with tumstatin via local injection. The rabbits were randomly assigned into tumstatin treated group, tumstatin preventive treated group, triamcinolone ace-tonide treated group and saline control group. Twenty-one days after the injection, the scar tissues on the bilateral ears of each rabbit were resected and then divided into four equal parts. Vessel density changes were determined by histological examination and CD34 im-munohistochemistry. Endothelial cells and fibroblasts apoptosis were detected. Results The Vessel density, viability of endothelium cells and the number of fibroblasts in the tumstatin treated group and the tumstatin preventive treated group was significantly lower than that in the triamcinolone acetonide treated group and saline control group, and the difference is statistically significant. Conclusions In conclusion, Compared with triamcinolone acetonide, tumstatin works better in inhibiting the growth of hypertrophic scars, which provides a preliminary theoretical basis for the feasibility of treatment and prevention of hypertrophic scar.