丹参川芎嗪对兔耳增生性瘢痕的抑制作用研究

摘要

Objective To observe the inhibitory effect of Salviae Miltiorrhizae and Ligustrazine Hydrochloride Injection on hypertrophic scars of rabbit ears. Methods Totally 16 healthy Japanese white big-ear rabbits were selected to establish animal model of hyper-trophic scars of rabbit ears. After the wound epithelialization ( 21 d after injury ) , the rabbits were randomly divided into 4 groups, 4 rabbits in each group. The control group ( group A ) was partially injected with normal saline, and the experimental groups ( group B, C, D ) were partially injected with low, medium and high concentration of Salviae Miltiorrhizae and Ligustrazine Hydrochloride Injection. Scar thickness was measured on the 32nd and 56th day after injury, and HE staining was routinely performed, the content of HPr in scar tissue was determined by chloramine-T method. The expression and changes of proliferating cell nuclear antigen ( PCNA ) in scar tissue were observed by immunohistochemistry. Results In the experimental group, the thickness of scar, the content of HPr and the positive expression rate of PCNA were negatively correlated with the drug concentration ( P < 0. 05 ) . The collagen content and PCNA protein level in the scar tissue of the experimental group were significantly decreased, showing a dose-response relationship, and the difference was statistically significant compared with the control group ( P < 0. 01 ) . In the experimental group, there was significantdifferenceamong the subgroups on the 32nd and 56th days after injury ( P < 0. 01 ) . Conclusion Salviae Miltiorrhizae and Ligustrazine Hydrochloride Injection can inhibit the proliferation of hypertrophic scar tissue in rabbit ears by reducing the level of collagen synthesis and the expression of PCNA protein, which can provide new options for the treatment of hypertrophic scars.%目的 观察丹参川芎嗪注射液对兔耳增生性瘢痕的抑制作用.方法 取日本大耳白兔16只,建立兔耳增生性瘢痕动物模型.待创面上皮化(伤后21 d)后将其随机分为4组,每组4只,对照组(A组)局部注射生理盐水,实验组(B,C,D组)分别局部注射低、中、高质量浓度的丹参川芎嗪注射液.于伤后32 d和56 d测量瘢痕厚度,并常规做HE染色,氯胺-T法检测瘢痕组织中羟脯氨酸(HPr)的含量变化;免疫组化观察瘢痕组织中增殖细胞核抗原蛋白(PCNA)的表达及变化.结果 实验组中瘢痕厚度、HPr的含量及PCNA的阳性表达率与药物质量浓度呈负相关(P<0.05),瘢痕组织中胶原含量和PCNA蛋白水平均明显减少,呈剂量效应关系,与对照组比较,差异有统计学意义(P<0.01),伤后32 d和56 d实验组各亚组间差异有统计学意义(P<0.01).结论 丹参川芎嗪注射液通过减少胶原的合成水平,减少PCNA蛋白的表达,从而抑制兔耳增生性瘢痕组织增生,可为治疗增生性瘢痕提供新选择.