早期胃癌中Runx-3及β-catenin蛋白的表达及临床意义

摘要

目的 探讨Runx-3及β-catenin蛋白在早期胃癌组织中的表达及两者表达与临床病理特征的关系.方法 应用免疫组化EnVision两步法分别检测Runx-3和β-catenin蛋白在30例正常胃黏膜和49例早期胃癌组织中的表达.结果 Runx-3蛋白在正常胃黏膜中的阳性率(86.67％)明显高于早期胃癌组织(34.69％) (P ＜0.05);β-catenin蛋白在正常胃黏膜细胞膜中的阳性率(100％)显著高于早期胃癌组织(57.14％)(P＜0.05),但其异位表达率(0)显著低于早期胃癌组织(75.51％)(P＜0.05).早期胃癌组织中Runx-3及β-catenin蛋白表达与患者性别、年龄、肿块大小等均无关(P＞0.05),但有脉管侵犯的早期胃癌组织中Runx-3蛋白细胞核阳性率显著高于无脉管侵犯者(P＜0.05);有脉管侵犯的早期胃癌组织中β-catenin蛋白细胞膜表达缺失率显著高于无脉管侵犯者(P＜0.05).结论 Runx-3蛋白在早期胃癌组织中的表达明显减少,提示其可能作为胃癌早期诊断的参考指标;β-catenin蛋白在早期胃癌组织中膜表达缺失而存在核/质异位表达,在脉管侵犯的早期胃癌组织中β-catenin蛋白膜表达缺失,而Runx-3蛋白细胞核阳性表达增加,提示两者与早期胃癌的脉管侵犯相关;联合检测Runx-3蛋白及β-catenin蛋白有助于早期胃癌的诊断.%Purpose To evaluate the relationship between the expression of Runx-3 and β-catenin protein in EGC with the clinical factors.Methods Immunohistochemistry (IHC) was used to detect the expression of Runx-3 and β-catenin protein in 30 cases of normal gastric mucosa and 49 cases of EGC.Results The expression rate of Runx-3 protein in normal gastric mucosa (86.67％) was significantly higher than that in EGC tissues (34.69％) (P ＜ 0.05).β-catenin staining was strongly positive (100％) in normal gastric mucosa compared with that in EGC (57.14％) (P ＜ 0.05),while the abnormal expression rate of β-catenin (0) was significantly lower than that in EGC tissues (75.51％) (P ＜ 0.05).No correlation was found between the expression of Runx-3 and β-catenin protein with the clinicopathological factors,including sex,age,and tumor size except vessel invasion.Conclusion The reduced expression of Runx-3 in EGC indicates that it may be used as a favorable marker for the diagnosis of EGC.The loss of β-catenin protein membrane expression while ectopic expressed on nuclear or cytoplasm in EGC and the membrane expression of β-catenin protein is completely absent in the early stage of vascular invasion suggesting that the expression of β-catenin protein is closely related to the occurrence and development of EGC.A join-detection of Runx-3 and β-catenin expression will be helpful for the diagnosis of EGC.