基于甲酸和氢氧化钠的衍生移动反应界面的数值计算与实验验证

摘要

A model of derivatized moving reaction boundary( MRB)is formulated from original MRB formed with formic buffer( phase α)and sodium hydroxide( phase γ). The model shows the formation of a new phase ( phase β)with high pH value originated from phase γ,derivatized MRB created with phases α and β,as well as stationary boundary formed between phases β and γ. To demonstrate the validity of this model,the theoreti-cal and numerical procedures are advanced. At the same time,the experimental procedures are also developed relied on a capillary electrophoresis( CE)and a home-made apparatus. There are evident systemic errors and low agreements between the experimental results and theoretical calculation with the original model of MRB de-veloped in our previous papers. However,there are much high agreements between the experiments and theo-retical computation relied on the model of derivatized MRB developed herein. The model of derivatized MRB to-gether with the relative theoretical and experimental procedures holds evident significance for the design of the new separation and compressive techniques of samples used widely in electrophoresis including CE.%在甲酸（α相）和氢氧化钠（γ相）缓冲液形成的移动反应界面的基础上，提出了一种衍生移动反应界面模型。模型表明在α相和γ相之间会形成一个新的β相，β相和α相形成衍生移动反应界面，β相和γ相形成移动界面。为了验证该模型的有效性，该文给出了相关理论及数值推导过程。此外，基于毛细管电泳和自制装置进行了相关实验。结果表明，若使用以前的移动反应界面，实验结果与理论计算存在较大误差，而采用该文提出的衍生移动反应界面，实验数据与理论计算结果高度一致。该文提出的衍生移动反应界面理论及模型对于电泳，特别是毛细管电泳中样品的分离与富集具有重要的意义。