Objective To analyze RNA oxidative damage biomarker of urine 8-oxo-Gsn , different types of coronary heart disease (CHD ) and numbers of coronary lesion vessels in patients with coronary heart disease.Methods 422 patients with chest pain were enrolledin this study. All subjects underwent coronary angiography (CAG )and were divided into control group (non-CHD group) ,and CHD group ,then the subjects in CHD group were divided into 3 subgroups according to CAG results ,clinical symptoms and characteristic :SA ,UA or AMI group. And also be divided into 3 subgroups according to thenumbers of coronary lesion vessels :single ,doubleand triple vessels lesion group.Clean urine specimens were collected before the patients accepting CAG. The urine 8-oxo-Gsnwasdetected using an ID-LC-MS/MS method , and corrected by urine creatinine concentration. Urine 8-oxo-Gsn were compared using SPSS 19.0. Positive rate between healthy subjects and CHD patients of different types were also compared.Results Average urine 8-oxo-Gsn (μmol/mol creatinine) of group C ,SA ,UA ,and MI were (2.875 ± 0.609) ,(3.458 ± 1.261) ,(3.464 ± 1.121) ,(3.640 ± 1.339 ). The difference of urine 8-oxo-Gsn between different types of CHD was statistically significant (P< 0.01) ,and there was statistically significant difference between every two groups ;Average urine 8-oxo-Gsn (μmol/mol creatinine ) of subgroups S ,D ,and T were (3.458 ± 1.261) ,(3.464 ± 1.121) ,(3.640 ± 1.339 ).Difference between lesion vesselsgroups was of statistical significance(P< 0.01) ,and there was no statistically difference between every two groups ;Positive rate of urine 8-oxo-Gsn between CHD and healthy patients was statistically different ( P < 0.01 ). Conclusions Both urine 8-oxo-Gsn level and positive rate in CHD subjects were significantly elevated ;Difference of urine 8-oxo-Gsn in subjects with different coronary lesion vessel numbers were not that significant ;Urine 8-oxo-Gsn may be a new biomarker indicating the elevated oxidative stress level in CHD patients.%目的：分析 RNA 氧化损伤标志物尿8-oxo-Gsn 与冠心病类型、冠脉病变支数冠心病类型、冠脉病变支数的关系。方法选择以胸痛入院的患者422例。将所有患者分为非冠心病组（C）和冠心病组，并根据冠心病组患者的临床特点将其分为三个亚组：稳定型心绞痛组（SA），不稳定型心绞痛组（UA）及急性心肌梗死组（MI）；根据冠状动脉病变支数将冠心病组患者分为三个亚组：单支病变（S）、双支病变（D）和三支病变组（T）。收集所有入组患者在接受造影前的清洁尿标本，采用同位素稀释高效液相-串联质谱法（ID-LC-MS／MS）检测尿8-oxo-Gsn ，并用尿肌酐浓度予以矫正。应用SPSS 19.0进行统计分析，比较不同组间尿8-oxo-Gsn 的差异及各组患者尿8-oxo-Gsn 阳性率的差异。结果 C 与 SA 、UA 、MI 组的尿8-oxo-Gsn（单位μmol／mol creatinine）的平均值分别为（2.875±0.6097）、（3.285±1.031）、（3.433±1.175）、（4.224±1.557），不同类型冠心病组尿8-oxo-Gsn 水平的差异有统计学意义（P＜0.01），每两组间差异有统计学意义（均为 P＜0.05）；S 、D 和 T 组患者的尿8-oxo-Gsn（单位μmol／mol creatinine）的平均值分别为（3.458±1.261）、（3.464±1.121）、（3.640±1.339），不同病变支数组尿8-oxo-Gsn 水平的差异有统计学意义（P ＜0.01），每两组间差异无统计学意义（均为 P＜0.05）；尿8-oxo-Gsn 阳性率在健康人及不同类型冠心病患者中的差异有统计学意义（P＜0.01）。结论冠心病患者尿8-oxo-Gsn 水平和阳性率较非冠心病者明显升高；尿8-oxo-Gsn 在不同冠脉病支数患者中的变化不显著。尿8-oxo-Gsn 或可作为一种潜在的生物标志物指示冠心病患者体内氧化应激水平的升高。
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