多拷贝策略在小肽表达中的应用

摘要

基因工程技术已经在大分子多肽表达上得到了广泛的应用.但是小分子多肽不稳定且易降解,使其表达后很难检测和纯化.多拷贝策略是将目的基因或是含有目的基因的表达盒首尾串联,而串联构建多拷贝表达载体是目前解决小分子多肽表达量少的有效方法.总结和比较非对称粘性末端互补法、接头连接法、同尾酶法和表达盒串联法在多肽表达方面的应用情况,为小分子多肽的体外表达提供方法和思路.%The technology of genetic engineering has been widely used to express macromolecules such as enzymes.However,it is difficult to detect and purify the micromolecules such as small peptides, because of their instability and degradability.Construction of multi-copy recombinant expression plasmid can be achieved by inserting multiple target genes or expression cassette containing target genes with the same orientation into expression vector.This is effective to increase the expression level of small peptides.In this article we described four methods in order to provide some optional methods and ideas for the expression of active small peptides.