升麻增效口腔溃疡散治疗口腔溃疡大鼠模型的研究

摘要

目的:研究升麻对口腔溃疡散治疗口腔溃疡大鼠增效作用及机制.方法:将大鼠分为空白组、模型组、溃疡散组、溃疡散+升麻组,除空白组外,以NaOH晶体化学烧灼法建立溃疡模型,检测各组溃疡面积、愈合率,HE染色法观察组织病理学变化,ELISA法检测IFN-γ和1L-6含量,Western-blot检测MMP-2、MMP-9蛋白表达.结果:造模大鼠溃疡明显,周围组织红肿;给药后溃疡面积缩小,其中口腔溃疡散联合升麻组在溃疡面积、IFN-γ、IL-6和MMP-2、MMP-9表达水平上较单独用药组均显著降低.结论:升麻增效口腔溃疡散发挥治疗作用,其机制可能与抑制炎性介质的释放以及下调MMP-2、MMP-9蛋白表达有关.%Objective:To study the treatment of oral ulcer powder Cimicifuga experimental oral ulcer in rats and its partly mechanisms of synergistic effect.Methods:The rats were divided into blank group,oral ulcer film group,ulcer powder group,ulcer powder combined + cohosh group.Those groups were established by film NaOH crystal chemical cauterization except the blank group.Then HE method for oral mucosal tissue staining to observe the pathological changes,the detection area rats of group size,healing rate changes.ELISA method was used to detect the changes of IFN-γand IL-6 detection.Western-blot method was used to detect the expression of MMP-2,9.Results:After modeling,ulcer in the rats of model group was round or oval,the surrounding tissue swelling;the ulcer area was gradually reduced after administration.In the ulcer area,IFN-γ,the levels of IL-6 and MMP-2,MMP-9 expression level,the oral ulcers scattered combined with cohosh group were significantly lower than the single drug group.Conclusion:Oral ulcer powder combined with Rhizoma Cimicifugae synergy in the treatment of oral ulcer,and its mechanism may release and inhibition of inflammatory mediators and down regulated the expression of MMP-2,MMP-9.