Preparation of reconstituted rat embryos, useful as study models and for production of therapeutic proteins, by injecting nucleus into an ovocyte that has been stored in a maintenance medium

摘要

Preparation of reconstituted rat embryos (A) by nuclear transfer comprises inserting the nucleus of a rat cell into an ovocyte, collected previously and stored in vitro in maintenance medium, then activation of the reconstituted ovocyte. Preparation of reconstituted rat embryos (A) by nuclear transfer comprises inserting the nucleus of a rat cell into an ovocyte, collected previously and stored in vitro in maintenance medium, then activation of the reconstituted ovocyte. The new features are that (a) the ovocyte is maintained in the metaphase stage of the second meiotic division in a medium that contains a reversible inhibitor (I) of the proteosome and (b) the reconstituted ovocyte is activated using a medium that contains an inducer of activation (II). Independent claims are also included for the following: (1) rats, as embryo, fetus, neonate or adult, and cells derived from them, produced by the new process; and (2) in vitro process for cloning rats by nuclear transfer that comprises or includes the new process.